Abstract
A method is reported for recognizing MCF-7 human breast carcinoma cells based on silica-encapsulated nanoparticles modified with aminophenylboronic acid which can recognize sialic acid on cell surfaces. Gold@rhodamine B nanoparticles were coated with aminophenylboronic acid and used to capture MCF-7 cells. It is found that the presence of gold NPs was favorable to prepare nanoparticles easily and that they were extraordinarily biocompatible with MCF-7 cells. The experimental results confirmed that the nanoparticles can be used to target breast carcinoma cells using HS 578Bst normal breast cells as the negative control. The MCF-7 cells were imaged by laser scanning microscopy and showed strong red fluorescence in dark field. An MTT test revealed an 82 % viability of cells when 50 mg · mL−1 fluorescent probe was used in the incubation experiments. The results exhibited that the NPs are innocuous and stable. In our perception, the method has a larege potential for early diagnosis of breast cancer due to high affinity between nanoparticles and the breast carcinoma cells.
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Acknowledgments
This work was supported by the Project of the National Science Foundation of People’s Repubic of China (21275100), Shanghai Leading Academic Discipline Project (S30406) and Key Laboratory of Resource Chemistry of Ministry of Education.
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Wu, L., Yan, Y., Gao, P. et al. Recognition of MCF-7 human breast carcinoma cells using silica-encapsulated fluorescent nanoparticles modified with aminophenylboronic acid. Microchim Acta 183, 1115–1122 (2016). https://doi.org/10.1007/s00604-015-1736-9
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DOI: https://doi.org/10.1007/s00604-015-1736-9