Skip to main content
SpringerLink
Log in

Determination of indometacin and acemetacin in human urine via reduced graphene oxide - based pipette tip solid-phase extraction coupled to HPLC

  • Original Paper
  • Published:
Microchimica Acta Aims and scope Submit manuscript

Abstract

A rapid method for solid-phase extraction (SPE) and quantitation of the non-steroidal anti-inflammatory drugs indometacin and acemetacin in urine was developed. SPE is based in of the use of a reduced graphene oxide (rGO) in a pipette tip, and separation and quantitation is based on HPLC with a Promosil C18 column and UV detection. The amount of sorbent, types and volumes of washing solvent and elution solvent were optimized. The results showed that the use of the rGO sorbent and a miniaturized pipette tip cartridge are distinctly superior to other sorbents including multiwalled carbon nanotubes and conventional SPE cartridges. Linear responses to indometacin and acemetacin are found for the 0.1–50 μg mL−1 concentration ranges, recoveries (at three spiking levels) range from 89.6 % to 95.7 %, and the relative standard deviations are ≤6.4 %.

A rapid method for solid-phase extraction (SPE) and quantitation of the non-steroidal anti-inflammatory drugs indometacin and acemetacin in urine was developed. SPE is based on the use of reduced graphene oxide (rGO) in a pipette tip, and separation and quantitation is based on HPLC with a Promosil C18 column and UV detection.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Liu Q, Shi J, Jiang G (2012) Application of graphene in analytical sample preparation. Trends Anal Chem 37:1–11

    Article  Google Scholar 

  2. Sitko R, Zawisza B, Malicka E (2013) Graphene as a new sorbent in analytical chemistry. Trends Anal Chem 51:33–43

    Article  CAS  Google Scholar 

  3. Aziz-Zanjani MO, Mehdinia A (2014) A review on procedures for the preparation of coatings for solid phase microextraction. Microchim Acta 181:1169–1190

    Article  CAS  Google Scholar 

  4. Sundramoorthy AK, Gunasekaran S (2014) Applications of graphene in quality assurance and safety of food. Trends Anal Chem 60:36–53

    Article  CAS  Google Scholar 

  5. Sreeprasad TS, Maliyekkal SM, Lisha KP, Pradeep P (2011) Reduced graphene oxide–metal/metal oxide composites: facile synthesis and application in water purification. J Hazard Mater 186:921–931

    Article  CAS  Google Scholar 

  6. Liu Q, Shi J, Zeng L, Wang T, Cai Y, Jiang G (2011) Evaluation of graphene as an advantageous adsorbent for solid-phase extraction with chlorophenols as model analytes. J. Chromatogr. A 1218:197–204

    Article  CAS  Google Scholar 

  7. Huang K, Jing Q, Wei C, Wu Y (2011) Spectrofluorimetric determination of glutathione in human plasma by solid-phase extraction using graphene as adsorbent. Spectrochim. Acta A 79:1860–1865

    Article  CAS  Google Scholar 

  8. Wang Y, Gao S, Zang X, Li J, Ma J (2012) Graphene-based solid-phase extraction combined with flame atomic absorption spectrometry for a sensitive determination of trace amounts of lead in environmental water and vegetable samples. Anal Chim Acta 716:112–118

    Article  CAS  Google Scholar 

  9. Zhang S, Du Z, Li G (2011) Layer-by-layer fabrication of chemical-bonded graphene coating for solid-phase microextraction. Anal Chem 83:7531–7541

    Article  CAS  Google Scholar 

  10. Wu Q, Zhao G, Feng C, Wang C, Wang Z (2011) Preparation of a graphene-based magnetic nanocomposite for the extraction of carbamate pesticides from environmental water samples. J Chromatogr A 1218:7936–7942

    Article  CAS  Google Scholar 

  11. Wu Q, Liu M, Ma X, Wang W, Wang C, Zang X, Wang Z (2012) Extraction of phthalate esters from water and beverages using a graphene-based magnetic nanocomposite prior to their determination by HPLC. Microchim Acta 177:23–30

    Article  CAS  Google Scholar 

  12. Sun N, Han Y, Yan H, Song Y (2014) A self-assembly pipette tip graphene solid-phase extraction coupled with liquid chromatography for the determination of three sulfonamides in environmental water. Anal Chim Acta 810:25–31

    Article  CAS  Google Scholar 

  13. Molina-García L, Cordóva MLF, Ruiz-Medina A (2010) Sensitive determination of indomethacin in pharmaceuticals and urine by sequential injection analysis and optosensing. J AOAC Int 93:1443–1449

    Google Scholar 

  14. Abou-Ghannam G, Usta IM, Nassar AH (2012) Indomethacin in pregnancy: applications and safety. Am J Perinatol 29:175–186

    Article  Google Scholar 

  15. Michail K, Moneeb MS (2011) Determination of methotrexate and indomethacin in urine using SPE-LC-DAD after derivatization. J Pharm Biomed Anal 55:317–324

    Article  CAS  Google Scholar 

  16. Bakkali A, Corta E, Berrueta LA, Gallo B, Vicente F (1999) Study of the solid-phase extraction of diclofenac sodium, indomethacin and phenylbutazone for their analysis in human urine by liquid chromatography. J Chromatogr B 729:139–145

    Article  CAS  Google Scholar 

  17. Singh AK, Jang Y, Mishra U, Granley K (1991) Simultaneous analysis of flunixin, naproxen, ethacrynic acid, indomethacin, phenylbutazone, mefenamic acid and thiosalicylic acid in plasma and urine by high-performance liquid chromatography and gas chromatography-mass spectrometry. J Chromatogr B 568:351–361

    Article  CAS  Google Scholar 

  18. Nishioka R, Harimoto T, Umeda I, Yamamoto S, Ōi N (1990) Improved procedure for determination of indomethacin in plasma by capillary gas chromatography after solid-phase extraction. J Chromatogr B 526:210–214

    Article  CAS  Google Scholar 

  19. Makino K, Yano T, Maiguma T, Teshima D, Sendo T, Itoh Y, Oishi R (2003) A rapid and simultaneous determination of several analgesic antiinflammatory agents by capillary zone electrophoresis. Ther Drug Monit 25:574–580

    Article  CAS  Google Scholar 

  20. Chen Y, Wu S (2005) Capillary zone electrophoresis for simultaneous determination of seven nonsteroidal anti-inflammatory drugs in pharmaceuticals. Anal Bioanal Chem 381:907–912

    Article  CAS  Google Scholar 

  21. Yang H, Li F, Shan C, Han D, Zhang Q, Niu L, Ivaska A (2009) Covalent functionalization of chemically converted graphene sheets via silane and its reinforcement. J Mater Chem 19:4632–4638

  22. Li Z, Zhao L, Liang N, Chen H, Hou X (2014) Simultaneous determination of seven residual pharmaceuticals in wastewater by solid-phase extraction and liquid chromatography coupled to tandem mass spectrometry with a switching ionization mode. Anal Methods 6:9045–9052

    Article  CAS  Google Scholar 

  23. Wille SMR, Maudens KE, Van Peteghem CH, Lambert WEE (2005) Development of a solid phase extraction for 13 ‘new’ generation antidepressants and their active metabolites for gas chromatographic-mass spectrometric analysis. J Chromatogr A 1098:19–29

    Article  CAS  Google Scholar 

  24. Lee J, Kim H, Jeong JC, Park ES, Hwang KW, Yang SJ, Jeong JH (2007) Determination of beraprost in human plasma by a high-performance liquid chromatography-tandem mass spectrometry. J Chromatogr B 859:229–233

    Article  CAS  Google Scholar 

  25. Yang T, Li YH, Wei S, Li Y, Deng A (2008) Development of a selective molecularly imprinted polymer-based solid-phase extraction for indomethacin from water samples. Anal Bioanal Chem 391:2905–2914

    Article  CAS  Google Scholar 

  26. Locatelli M, Ferrone V, Cifelli R, Barbacane RC, Carlucci G (2014) MicroExtraction by packed sorbent and HPLC determination of seven non-steroidal anti-inflammatory drugs in human plasma and urine. J. Chromatogr. A 1367:1–8

    Article  CAS  Google Scholar 

  27. Suenami K, Lim LW, Takeuchi T, Sasajima Y, Sato K, Takekoshi Y, Kanno S (2006) Surfactant coated magnetic nanoparticle-based solid-phase extraction coupled with spectrophotometric detection for determination of ultra-trace amounts of indomethacin in biological fluids. Anal Bioanal Chem 384:1501–1505

    Article  CAS  Google Scholar 

  28. Wang X, Vernikovskaya DI, Nanovskaya TN, Rytting E, Hankins GDV, Ahmed MS (2013) A liquid chromatography method with single quadrupole mass spectrometry for quantitative determination of indomethacin in maternal plasma and urine of pregnant patients. J Pharm Biomed Anal 78:123–128

    Article  Google Scholar 

Download references

Acknowledgments

The project was sponsored by National Natural Science Foundation of China (21575033), Natural Science Foundation of Hebei Province (B2015201132, B2014201089) and Technology Foundation for Selected Overseas Scholar (C201400301).

Author information

Authors and Affiliations

  1. Department of Preventive Medicine and Health Management, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Hebei University, Baoding, 071002, China

    Yanan Yuan, Ning Sun, Hongyuan Yan & Dandan Han

  2. Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of Pharmacy, Hebei University, Baoding, 071002, China

    Yanan Yuan, Ning Sun & Hongyuan Yan

  3. Department of Chemistry and Chemical Engineering, Inha University, Incheon, 402751, South Korea

    Kyung Ho Row

Authors
  1. Yanan Yuan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ning Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hongyuan Yan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Dandan Han
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Kyung Ho Row
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hongyuan Yan.

Electronic supplementary material

ESM 1

(DOC 987 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yuan, Y., Sun, N., Yan, H. et al. Determination of indometacin and acemetacin in human urine via reduced graphene oxide - based pipette tip solid-phase extraction coupled to HPLC. Microchim Acta 183, 799–804 (2016). https://doi.org/10.1007/s00604-015-1711-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00604-015-1711-5

Keywords

Search

Navigation