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Amperometric oxygen biosensor based on hemoglobin encapsulated in nanosized grafted starch particles

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Abstract

The authors describe a nanosized oxygen biosensor based on grafted starch particles with encapsulated hemoglobin (GS-Hb). Transmission electron microscopy showed the GS-Hb particles to have a typical diameter of 573 nm. UV–vis absorption spectra showed that the hemoglobin in GS-Hb retains its biological activity in terms of carrying and releasing oxygen. Cyclic voltammetric studies were performed with GS-Hb particles placed in a chitosan matrix on a glassy carbon electrode, revealing a pair of nearly reversible redox peaks. At a working potential of −0.40 V (vs. SCE), a linear response is found over the 0.97 μM to 0.35 mM oxygen concentration range, and the lower detection limit is 0.32 μM (~5 ppb; at an S/N ratio of 3). This range is wider than that produced when using red blood cells. In addition, the GS-Hb particles have a better oxygen-carrying ability, which may pave the way to the creation of oxygen carriers for use in transfusions.

A glassy carbon electrode modified with hemoglobin in starch particles in a chitosan matrix shows excellent amperometric response to oxygen and also displays improved oxygen-carrying capability (compared to red blood cells).

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (Grant numbers: 21475070; 21175075), the Natural Science Foundation of Jiangsu Province (Grant number: BK2011047, BK2012651), the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), and the 226 Scientific Research Project of Nantong City.

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    Authors and Affiliations

    1. School of Public Health, Institute of Analytical Chemistry for Life Science, Nantong University, Nantong, 226019, China

      Xiaojun Liu, Zhongqin Pan, Zenglin Dong, Yannan Lu, Tingting Wu, Ning Bao & Haiying Gu

    2. Affiliated Hospital, Nantong University, Nantong, 226001, China

      Hong He

    3. Department of Clinical Laboratory, Nantong Tumor Hospital, Nantong, 226361, China

      Qiaoling Sun

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    1. Xiaojun Liu
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    Correspondence to Hong He or Haiying Gu.

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    Xiaojun Liu and Zhongqin Pan contributed equally to this work.

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    Liu, X., Pan, Z., Dong, Z. et al. Amperometric oxygen biosensor based on hemoglobin encapsulated in nanosized grafted starch particles. Microchim Acta 183, 353–359 (2016). https://doi.org/10.1007/s00604-015-1655-9

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