A nanocomposite consisting of graphene oxide and Fe3O4 magnetic nanoparticles for the extraction of flavonoids from tea, wine and urine samples
- 626 Downloads
We describe a single-step solvothermal method for the preparation of nanocomposites consisting of graphene oxide and Fe3O4 nanoparticles (GO/Fe3O4). This material is shown to be useful as a magnetic sorbent for the extraction of flavonoids from green tea, red wine, and urine samples. The nanocomposite is taking advantage of the high surface area of GO and the magnetic phase separation feature of the magnetic sorbent. The nanocomposite is recyclable and was applied to the extraction of flavonoids prior to their determination by HPLC. The effects of amount of surfactant, pH value of the sample solution, extraction time, and desorption condition on the extraction efficiency, and the regeneration conditions were optimized. The limits of detection for luteolin, quercetin and kaempferol range from 0.2 to 0.5 ng∙ mL−1 in urine, from 3.0 to 6.0 ng∙mL−1 in green tea, and from 1.0 to 2.5 ng∙mL−1 in red wine. The recoveries are between 82.0 and 101.4 %, with relative standard deviations of <9.3 %.
KeywordsGraphene oxide-Fe3O4 Nanocomposite Magnetic solid phase extraction Vibrating sample magnetometer Flavonoids High performance liquid chromatography HPLC
This work was supported by the National Natural Science Foundation of China (Grant No. 81402899), the Open Project of Key Laboratory of Modern Toxicology of the Ministry of Education (Grant No. NMUMT201404), National Basic Science Personal Training Fund (No. J0630858) and the Key Laboratory of Biomedical Functional Materials, China Pharmaceutical University.
- 8.Hadjmohammadi M, Karimiyan H, Sharifi V (2013) Hollow fibre-based liquid phase microextraction combined with high-performance liquid chromatography for the analysis of flavonoids in Echinophora platyloba DC and Mentha piperita. Food Chem 141(2):731–735. doi: 10.1016/j.foodchem.2013.02.083 CrossRefGoogle Scholar
- 9.de Vries JHM, Hollman PC, Meyboom S, Buysman MNCP, Zock PL, van Staveren WA, Katan MB (1998) Plasma concentrations and urinary excretion of the antioxidant flavonols quercetin and kaempferol as biomarkers for dietary intake. Am J Clin Nutr 68:60–65Google Scholar
- 10.Young JF, Nielsen SE, Haraldsdóttir J, Daneshvar B, Lauridsen ST, Knuthsen P, Crozier A, Sandström B, Dragsted LO (1999) Effect of fruit juice intake on urinary quercetin excretion and biomarkers of antioxidative status. Am J Clin Nutr 69:87–94Google Scholar
- 19.Han Q, Wang Z, Xia J, Chen S, Zhang X, Ding M (2012) Facile and tunable fabrication of Fe3O4/graphene oxide nanocomposites and their application in the magnetic solid-phase extraction of polycyclic aromatic hydrocarbons from environmental water samples. Talanta 101:388–395. doi: 10.1016/j.talanta.2012.09.046 CrossRefGoogle Scholar
- 23.Heidari H, Razmi H (2012) Multi-response optimization of magnetic solid phase extraction based on carbon coated Fe3O4 nanoparticles using desirability function approach for the determination of the organophosphorus pesticides in aquatic samples by HPLC-UV. Talanta 99:13–21. doi: 10.1016/j.talanta.2012.04.023 CrossRefGoogle Scholar
- 24.Meng J, Shi C, Wei B, Yu W, Deng C, Zhang X (2011) Preparation of Fe3O4@C@PANI magnetic microspheres for the extraction and analysis of phenolic compounds in water samples by gas chromatography–mass spectrometry. J Chromatogr A 1218(20):2841–2847. doi: 10.1016/j.chroma.2011.03.044 CrossRefGoogle Scholar
- 29.Wang W, Ma R, Wu Q, Wang C, Wang Z (2013) Fabrication of magnetic microsphere-confined graphene for the preconcentration of some phthalate esters from environmental water and soybean milk samples followed by their determination by HPLC. Talanta 109:133–140. doi: 10.1016/j.talanta.2013.02.008 CrossRefGoogle Scholar