Selective assembly of Au-Fe3O4 nanoparticle hetero-dimers
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Hetero-dimeric magnetic nanoparticles of the type Au-Fe3O4 have been synthesised from separately prepared, differently shaped (spheres and cubes), monodisperse nanoparticles. This synthesis was achieved by the following steps: (a) Mono-functionalising each type of nanoparticles with aldehyde functional groups through a solid support approach, where nanoparticle decorated silica nanoparticles were fabricated as an intermediate step; (b) Derivatising the functional faces with complementary functionalities (e.g. amines and carboxylic acids); (c) Dimerising the two types of particles via amide bond formation. The resulting hetero-dimers were characterised by high-resolution TEM, Fourier transform IR spectroscopy and other appropriate methods.
KeywordsGold nanoparticles Iron oxide nanoparticles Dimers Hetero-dimers Mono-functionalisation
Sketches and simulations of complex nano-machines have been published since the advent of nano-sciences in the early ‘90s. However, the covalent coupling of two different, separately synthesised nanoparticles to form a simple hetero-dimer has turned out to be extremely difficult. In this article, we show a straightforward and generic strategy to accomplish this initial step into nano-architecture design by extending a solid support synthesis method we have developed earlier for the preparation of homo-dimers .
The synthesis of high-quality nanocrystals (or nanoparticles) has reached a level where monodisperse and well-defined particles can be made of nearly any material. This includes metallic, semi-conducting and magnetic nanoparticles , which have been synthesised and used for numerous (bio-)analytical applications such as, magnetic contrast enhancement and sensing [3, 4, 5], bio-imaging [3, 6, 7], fluorescent marking [8, 9], hyperthermia [10, 11], and catalysis . Interestingly, some analytical methods are based on the dimerisation or controlled coagulation of individual nanoparticles [13, 14]. It is known that the properties and performance of nanoparticles are strongly affected by the shape, size, crystalline structure, as well as the monodispersity of the materials [15, 16, 17]. Although it is possible to tune the properties of nanoparticles by modifying these attributes, there are limits in engineering chemical and physical properties. To overcome these restrictions, seed-mediated growth methods have been developed for a number of materials, resulting in multi-functional particles [18, 19]. While these techniques are advantageous, they are severely limited in the choice of materials due to the degree of chemical compatibility required for the techniques (e.g. lattice mismatch, redox behaviour, and defect formation). The covalent linkage of two different nanoparticles constitutes the first step in extending the range of engineer-able properties and paves the road for higher complexity nano-systems.
Our method is substantially different from the seed-mediated methods mentioned above and biomolecule supported strategies (for example using deoxyribonucleic acid (DNA)) that led to controlled nanoparticle assemblies in the past [20, 21]. The mono-functionalisation strategy used in this work is grounded in modifying nanoparticles selectively at their point of contact to a much larger surface [1, 22, 23]. This strategy follows a similar approach to Merrifield peptide synthesis, thus Merrifield resin [24, 25] and a range of other commercial supports (such as polymer Wang resin ) were considered initially. However, none of the commercial supports were suitable for our strategy, as it was found that they tend to wrap the nanoparticles, impeding mono-functionalisation, and making it difficult or impossible to separate from the nanoparticles after the reaction. Silica nanoparticles (SiO2 NPs) were selected as a means of providing this rigid, stable, and high-surface area structural support and were successfully used for a different type of mono-functionalisation earlier .
Results and discussion
Silica nanoparticles with an average diameter of about 50 nm were synthesised via the Stöber method [27, 28, 29, 30] (a size histogram is available in the supporting information Figure S4). The silica particles were subsequently aminated with 3-aminopropylethoxysilane (APTES) and functionalisatized with a cleavable linker via ethyl (dimethylaminopropyl)carbodiimide / N-hydroxysuccinimide (EDC/NHS) conjugation. Tartaric acid (C4H6O6) was chosen as it can be cleaved by oxidation at its vicinal alcohol groups using sodium periodate (NaIO4) (supporting information, Figure S5). The dually carboxylate-terminated molecule also allows for further functionalization via EDC/NHS peptide bond formation.
The resulting functionalised silica nanoparticles were analysed by means of dynamic light scattering (DLS) and TEM (see Fig. 1, top) to monitor particle integrity. Fourier transform infrared spectroscopy (FTIR) confirmed the final linker had the desired structure as depicted above (details of the synthesis procedures and analytical data can be found in the supporting information).
At this stage, the nanoparticles still bore excess activated carboxylic groups on their surface. It is crucial to passivate these groups prior to cleavage in order to obtain mono-functional nanoparticles. We have explored two options at achieve this: first, reaction of the activated carboxylic groups with ethanolamine. Second, swift cleavage and dimerization before hydrolysis of the intermediate occurred to a significant degree (we will discuss this strategy in more detail later). Both options prepared the nanoparticles for the oxidative cleavage.
After cleavage, silica dissolution, and purification, both types of nanoparticles bore aldehyde groups clustered in the monofunctionalization ‘spot’ (confirmed by FTIR spectroscopy). In order to form hetero-dimers, the two types of nanoparticles have to be functionalised with different reactive groups. In principle, it would be sufficient to convert the functional spot of one type of nanoparticles to amines and then dimerise two different types by reductive amination (this would also make the passivation step discussed above obsolete). However, for the sake of generality, we also functionalised one type of nanoparticle with carboxylic and the other with primary amine groups for subsequent formation of an amide bond. Mono-functionalised Fe3O4 nanoparticles were reacted with glycine by reductive amination; the primary amine of glycine reacts with the aldehyde groups of the nanoparticles and forms a Schiff base. Schiff bases are unstable and readily reversed by hydrolysis in aqueous solutions, therefore, they were reduced to secondary amines by addition of sodium cyanoborohydride (NaCNBH3) . The spectrum in Fig. 4d confirms the formation of the secondary amine, mainly by the N-H bending vibration at 1493 cm−1. Gold nanoparticles were reacted with an excess of ethylenediamine (EDA) in the same manner, which resulted in amine mono-functionalised particles.
Au-Fe3O4 nanoparticle hetero-dimers were synthesised by dispersing both particles types in the same solution and coupling with the EDC/NHS conjugation method discussed above. Through the conjugation, carboxylate groups of Fe3O4 nanoparticles were reacted with amine groups of gold nanoparticles and formed amide bonds.
XRD, UV–vis and further FTIR analysis were carried out to confirm the formation of heterodimer of Au-Fe3O4 nanoparticles (data are shown in the supporting information).
In this work, we have synthesised hetero-dimer nanoparticles using a solid support approach by first creating a mono-functional surface for both designated nanoparticles (Fe3O4 and gold Nanoparticles). We have shown that the attachment of the nanoparticles onto the solid support and passivation steps were crucial as this will dictate whether or not a point of contact (mono-functional group) has been created. We have established a generic way to couple two dissimilar (in regards to their chemical, physical properties and core materials) and separately prepared nanoparticles covalently. The creation of hetero-dimer nanoparticles from simple nanoparticle building blocks represents the first step towards controlled assembly of complex nano-architectures. Furthermore, nanoparticle hetero-dimers have many applications in their own right, for example as multimodal contrast agents for bioimaging.
Great appreciation goes to Dr. Amelia Liu from Monash Centre for Electron Microscopy, Melbourne, Australia for helping us with HRTEM and STEM. Part of this research was also supported by the Australian Microscopy and Microanalysis Research Facility (AMMRF). This research was also conducted and funded in part by the Australian Research Council Centre of Excellence in Convergent Bio-Nano Science and Technology (project number CE140100036). A further part of this work was performed at the South Australian node of the Australian National Fabrication Facility (ANFF), a company established under the National Collaborative Research Infrastructure Strategy to provide nano and micro-fabrication facilities for Australia’s researchers.
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