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A nanocomposite consisting of plasma-polymerized propargylamine and graphene for use in DNA sensing

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Abstract

We report on a novel graphene-based nanoarchitecture modified with plasma-polymerized propargylamine (G-PpPG) and its application in electrochemical sensors for DNA. Films of G-PpPG were characterized by X-ray photoelectron spectroscopy and electrochemical impedance spectroscopy. The presence of graphene enhances the electrochemical activity of the films, and the high density of amino groups (deposited at a low plasma input power) on their surface assists in the immobilization of probe DNA on the water-swollen polymeric network. By contrast, the degree of hybridization of the total complementary target DNA to the probe DNA remains unchanged when G-PpPG nanofilms prepared at higher input power. No substantial non-specific adsorption of totally mismatched target DNA on the polymer films is observed because of the complete coverage of the probe DNA. The detection limit for total complementary target DNA is approximately 1.84 nmol · L−1. The dynamic range extends from 0.1 to 1,000 nmol · L−1. The new nanocomposite may also be used to immobilize other probe DNA sequences, and this makes the approach potentially applicable to the detection of other oligomers.

Preparing the DNA sensor made from the graphene-based nanoarchitecture modified by using PpPG (G-PpPG) includes the following processes: (a) Modifying the Au electrode with the graphene nanosheet, (b) depositing the PpPG film onto the Au electrode coated with graphene, (c) immobilizing the probe DNA onto the G-PpPG film, and (d) hybridizing the MM0 target with the G-PpPG film immobilized with P1

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Acknowledgments

This work was supported by the Program for the National Natural Science Foundation of China (NSFC Account Nos. 51173172 and 21104070).

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Authors and Affiliations

  1. Henan Provincial Key Laboratory of Surface and Interface Science, Zhengzhou University of Light Industry, No. 166, Science Avenue, Zhengzhou, 450001, China

    Linghao He, Yuanchang Zhang, Shunli Liu, Shaoming Fang & Zhihong Zhang

  2. Henan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration, Zhengzhou University of Light Industry, No. 166, Science Avenue, Zhengzhou, 450001, China

    Shaoming Fang & Zhihong Zhang

Authors
  1. Linghao He
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  2. Yuanchang Zhang
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  3. Shunli Liu
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  4. Shaoming Fang
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  5. Zhihong Zhang
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Correspondence to Zhihong Zhang.

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He, L., Zhang, Y., Liu, S. et al. A nanocomposite consisting of plasma-polymerized propargylamine and graphene for use in DNA sensing. Microchim Acta 181, 1981–1989 (2014). https://doi.org/10.1007/s00604-014-1300-z

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  • DOI: https://doi.org/10.1007/s00604-014-1300-z

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