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Templated in-situ synthesis of gold nanoclusters conjugated to drug target bacterial enoyl-ACP reductase, and their application to the detection of mercury ions using a test stripe

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Abstract

Fluorescent gold nanoclusters (AuNCs) were synthesized using a drug target bacterial enoyl-ACP reductase (FabI) as a template. The physical and chemical properties of the AuNCs were studied by UV-vis absorption, fluorescence, X-ray photoelectron spectroscopy and TEM. The AuNCs-FabI conjugate was prepared by in situ reduction of tetrachloroaurate in the presence of FabI. The conjugated particles were loaded onto nylon membranes by taking advantage of the electrostatic interaction between the negatively charged AuNCs@FabI and the nylon film which is positively charged at pH 7.4. This results in the formation of a test stripe with sensor spots that can be used to detect Hg(II) ion in the 1 nM to 10 μM concentration range. The test stripes are simple, convenient, selective, sensitive, and can be quickly read out with bare eyes after illumination with a UV lamp.

Fluorescent gold nanoclusters (AuNCs) were synthesized using a drug target bacterial enoyl-ACP reductase (FabI) as a template. The synthesized AuNCs@FabI were loaded onto nylon membranes forming a paper-based sensor that can be used to detect Hg(II) ion in the 1 nM to 10 μM concentration range. The test stripes are simple, convenient, selective, sensitive, and can be quickly read out with bare eyes after illumination with a UV lamp.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 21003061, No. 21372097), Open Fund of the National Laboratory of Protein and Plant Gene Research at Peking University, and a CIMO grant from Finland.

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Authors and Affiliations

  1. State Key Laboratory of Supramolecular Structure and Materials, Jilin University, 2699 Qianjin Street, Changchun, 130012, People’s Republic of China

    Han Ding, Hongwei Li, Pengchang Liu, J. Kalervo Hiltunen, Yuqing Wu, Zhijun Chen & Jiacong Shen

  2. Faculty of Biochemistry and Molecular Medicine and Biocenter Oulu, University of Oulu, 90014, Oulu, Finland

    J. Kalervo Hiltunen & Zhijun Chen

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  1. Han Ding
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  2. Hongwei Li
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  3. Pengchang Liu
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  4. J. Kalervo Hiltunen
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  5. Yuqing Wu
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Correspondence to Zhijun Chen.

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Ding, H., Li, H., Liu, P. et al. Templated in-situ synthesis of gold nanoclusters conjugated to drug target bacterial enoyl-ACP reductase, and their application to the detection of mercury ions using a test stripe. Microchim Acta 181, 1029–1034 (2014). https://doi.org/10.1007/s00604-014-1201-1

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  • DOI: https://doi.org/10.1007/s00604-014-1201-1

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