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A porous silicon immunoassay platform for fluorometric determination of α-synuclein in human cerebrospinal fluid

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Abstract

Levels of total and/or oligomeric α-synuclein may be used as a biomarker tool to aid in the diagnosis and development of new disease-modifying therapies. We report here on a porous silicon antibody microarray for the fluorimetric determination of cerebrospinal fluid levels of total α-synuclein, a protein involved the pathology of Parkinson’s disease. The surface of porous silicon has a 3-dimensional macro- and nanoporous structure, and this offers a large binding capacity for capturing probe molecules. Porous silicon also warrants efficient immobilization of antibodies by surface adsorption, and does not require chemical immobilization. The platform requires 10 μL of cerebrospinal fluid, and each test requires 4 h for assay only (including immobilization of capturing antibody). The limit of detection is 35 pg mL−1 of α-synuclein in cerebrospinal fluid, and the dynamic analytical range extends from 0.01 to 100 ng·mL−1.

High antibody capturing capacity of porous silicon allows high density of antibody immobilization on the surface and make it possible enriching binding event to target protein (α−synuclein). Below shows SEM images of porous silicon surface and assayed microarray images.

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Acknowledgment

The Swedish Research Council-Linnaeus Grant, Bagadilico, The Swedish Research Council (grant no. 621-2009-5361; 2009-5361) and Korea- Swedish Research Cooporation Program (STINT) and STINT Institutional Grant: IG2010 2068. This project also supported by Korea Ministry of Environment as “EI project” (ERL E211-41003-0007-0), and Agency for Defence Development through Chemical and Biological Defence Research Center (2012-0126-005).

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Correspondence to Sangwook Lee or Thomas Laurell.

Additional information

Sangwook Lee and Edina Silajdžić equally contributed to the paper.

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Lee, S., Silajdžić, E., Yang, H. et al. A porous silicon immunoassay platform for fluorometric determination of α-synuclein in human cerebrospinal fluid. Microchim Acta 181, 1143–1149 (2014). https://doi.org/10.1007/s00604-014-1180-2

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Keywords

  • Porous silicon
  • Antibody microarray
  • α-synuclein
  • Parkinson’s disease