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Ultrasensitive detection of Dynabeads protein A using the giant magnetoimpedance effect

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Abstract

We report on a biosensing system for ultrasensitive detection of Dynabeads protein A (DPA) that employs the magnetoimpedance (GMI) effect. The system is capable of detecting DPA via magnetic signals in the form of a magnetoimpedance change. The GMI ratio shows distinctive changes because of the induced fringe field produced by the superparamagnetic Dynabeads. The GMI ratio undergoes an overall downturn at high frequencies, but the drop becomes smaller with increasing DPA concentration. This phenomenon has not been observed so far. At a concentration of 0.1 μg mL−1, the GMI ratio drops by 8.53 % at a frequency of 1.4 MHz. In other word: almost 90 Dynabeads can be detected. We believe that this novel scheme has a large potential in high-sensitivity and miniaturized immunoassays.

The GMI biosensing system is established for ultrasensitive detection of Dynabeads protein A. The rectangle nano-Au film is used for immobilization of the Dynabeads protein A. The longitudinal external field (He) is generated by a DC field source (0–125 Oe). The GMI biosensor is driven by AC with an amplitude of 10 mA (0.1–5 MHz).

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Acknowledgments

This work was supported by The National Natural Science Foundation of China (No. 61074168 and No.61273065),The National Science and Technology Support Program (2012BAK08B05), Basic Research Program of Science & Technology Committee of Shanghai (No. 09JC1408600), The Analytical and Testing Center in Shanghai Jiao Tong University, and The National Key Laboratory Research Found (No. 9140C790403110C7905).

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Authors and Affiliations

  1. Key Laboratory for Thin Film and Microfabrication Technology, Ministry of Education, Research Institute of Micro/Nanometer Science and Technology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China

    Tao Wang, Yong Zhou, Chong Lei, Jian Lei & Zhen Yang

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  1. Tao Wang
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  2. Yong Zhou
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  3. Chong Lei
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  4. Jian Lei
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  5. Zhen Yang
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Correspondence to Tao Wang or Yong Zhou.

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Wang, T., Zhou, Y., Lei, C. et al. Ultrasensitive detection of Dynabeads protein A using the giant magnetoimpedance effect. Microchim Acta 180, 1211–1216 (2013). https://doi.org/10.1007/s00604-013-1016-5

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  • DOI: https://doi.org/10.1007/s00604-013-1016-5

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