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Cation-exchange antibody labeling for simultaneous electrochemical detection of tumor markers CA15-3 and CA19-9

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Abstract

We report on a new kind of non-covalent multi-label electrochemical immunoassay that was applied to simultaneously quantify the tumor markers CA15-3 and CA19-9. The method employs a nanohybrid composed of an ionomer and conductive titanium dioxide nanoparticles that act as a matrix support for the antibodies. The two antibodies (anti-CA153 and anti-CA199) were labeled (a) with a cobaltous dipyridine complex, and (b) with methylene blue. Labeling is based on cation-exchange interaction rather than on covalent conjugation. The redox potentials of the two labels are separated by an interval of 0.3 V. The resulting sandwich-type immunosensor was read out by differential pulse voltammetry. The potential sites and currents of the two redox probes reflect the concentration of the two analytes. The two analytes were determined with a detection limit of 1.6 U mL−1 for CA19-9, and of 0.3 U mL−1 for CA15-3.

A new non-covalent multi-label electrochemical immunoassay was applied to simultaneously quantify dual tumor markers. DPV performed the identification and quantification of dual analytes on same electrode surface.

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Acknowledgments

This work was supported by the Key Project from Chongqing Science &Technology Commission of China (2009AC5031), and partially the National Natural Science Foundation of China (30670628 and 81101993).

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Authors and Affiliations

  1. Cancer Centre, Daping Hospital and Research Institute of Surgery, Third Military Medical University, Chongqing, 400042, People’s Republic of China

    Guangjie Wang, Yi Qing, Jinlu Shan, Feng Jin & Dong Wang

  2. Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, People’s Republic of China

    Ruo Yuan

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  1. Guangjie Wang
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  2. Yi Qing
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  3. Jinlu Shan
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  4. Feng Jin
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  5. Ruo Yuan
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  6. Dong Wang
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Corresponding author

Correspondence to Dong Wang.

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Guangjie Wang and Yi Qing contributed equally to this work.

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Wang, G., Qing, Y., Shan, J. et al. Cation-exchange antibody labeling for simultaneous electrochemical detection of tumor markers CA15-3 and CA19-9. Microchim Acta 180, 651–657 (2013). https://doi.org/10.1007/s00604-013-0973-z

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  • DOI: https://doi.org/10.1007/s00604-013-0973-z

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