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An enzymatic glucose biosensor based on a glassy carbon electrode modified with manganese dioxide nanowires

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Abstract

A glassy carbon electrode was modified with β-manganese dioxide (β-MnO2), and glucose oxidase (GOx) was immobilized on its surface. The β-MnO2 nanowires were prepared by a hydrothermal method and characterized by scanning electron microscopy and powder X-ray diffraction. They were then dispersed in Nafion solution and cast on the glassy carbon electrode (GCE) to form an electrode modified with β-MnO2 nanowires that exhibits improved sensitivity toward hydrogen peroxide. If GOx is immobilized in the surface, the β-MnO2 acts as a mediator, and Nafion as a polymer backbone. The fabrication process was characterized by electrochemical impedance spectroscopy, and the sensor and its materials were characterized by cyclic voltammetry and amperometry. The biosensor enables amperometric detection of glucose with a sensitivity of 38.2 μA · mM−1 · cm−2, and a response time of < 5 s. This study also demonstrates the feasibility of realizing inexpensive, reliable, and high-performance biosensors using MnO2 nanowires.

The sensitive determination of glucose was realized at a β-MnO2 NWs modified glassy carbon electrode by amperometry. The relatively fast, reproducible and low-cost manufacturing procedure suggests that it can offer an excellent platform for glucose oxidase-biosensing applications.

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Acknowledgment

The authors thank the National Natural Science Foundation of China (20975001, 21001004), Innovation experiment program for Anhui Normal University students (cxsy10022) for the financial supports.

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Authors and Affiliations

  1. College of Chemistry and Materials Science, Anhui Key Laboratory of Functional Molecular Solids, Anhui Normal University, No. 1 East Beijing Road, Wuhu, 241000, People’s Republic of China

    Li Zhang, Sheng-mei Yuan, Li-ming Yang, Zhen Fang & Guang-chao Zhao

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  1. Li Zhang
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  2. Sheng-mei Yuan
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  3. Li-ming Yang
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  4. Zhen Fang
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  5. Guang-chao Zhao
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Correspondence to Guang-chao Zhao.

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Zhang, L., Yuan, Sm., Yang, Lm. et al. An enzymatic glucose biosensor based on a glassy carbon electrode modified with manganese dioxide nanowires. Microchim Acta 180, 627–633 (2013). https://doi.org/10.1007/s00604-013-0968-9

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  • DOI: https://doi.org/10.1007/s00604-013-0968-9

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