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A study on the direct electrochemistry and electrocatalysis of microperoxidase-11 immobilized on a porous network-like gold film: Sensing of hydrogen peroxide

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Abstract

We have prepared porous and network-like nanofilms of gold by galvanic replacement of a layer of copper particles acting as a template. The films were first characterized by scanning electron microscopy and X-ray diffraction, and then modified with cysteamine so to enable the covalent immobilization of the enzyme microperoxidase-11. The immobilized enzyme undergoes direct electron transfer to the underlying electrodes, and the electrode displays high electrocatalytic activity towards the reduction of oxygen and hydrogen peroxide, respectively, owing to the largely enhanced electroactive surface of the porous gold film. The detection limit of H2O2 is 0.4 μM (3 S/N).

In this work, porous network-like Au films were prepared by galvanic replacement using Cu film as a sacrificial template. The cysteamine modified Au film was used to immobilize microperoxidase-11, which showed good stability and excellent electrochemical performance towards the reduction of O2 and H2O2, respectively

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Acknowledgments

This work was financially supported by the Nation Natural Science foundation of China (20805011, 20905021, 20905055, 51108424, 21175218 and 21275130), the Foundation of the Ministry of Education of China for Returned Scholars (A.J. Wang and J.J. Feng), and the opening funding of State Key Laboratory of Analytical Chemistry for Life Science of Nanjing University (SKLACLS1107 for J.J. Feng).

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Authors and Affiliations

  1. College of Chemistry and Life Science, College of Geography and Environmental Science, Zhejiang Normal University, Jinhua, 321004, China

    Qian-Li Zhang, Ai-Jun Wang, Zi-Yan Meng, Hong-Jun Lin & Jiu-Ju Feng

  2. School of Chemistry and Biological Engineering, Suzhou University of Science and Technology, Suzhou, 215009, China

    Qian-Li Zhang

  3. School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, 453007, China

    Ya-Hui Lu

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  1. Qian-Li Zhang
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Correspondence to Jiu-Ju Feng.

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Zhang, QL., Wang, AJ., Meng, ZY. et al. A study on the direct electrochemistry and electrocatalysis of microperoxidase-11 immobilized on a porous network-like gold film: Sensing of hydrogen peroxide. Microchim Acta 180, 581–587 (2013). https://doi.org/10.1007/s00604-013-0960-4

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  • DOI: https://doi.org/10.1007/s00604-013-0960-4

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