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Electrochemical immunoassay for subgroup J of avian leukosis viruses using a glassy carbon electrode modified with a film of poly (3-thiophene boronic acid), gold nanoparticles, graphene and immobilized antibody

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Abstract

We have modified a glassy carbon electrode (GCE) with a film of poly(3-thiophene boronic acid), gold nanoparticles and graphene, and an antibody (Ab) was immobilized on its surface through the covalent bond formed between the boronic acid group and the glycosyl groups of the Ab. Subgroup J of avian leukosis viruses (ALV-J) were electrochemically determined with the help of this electrode. There is a linear relationship between the electron transfer resistance (R et) and the concentration of ALV-J in the range from 527 to 3,162 TCID50⋅mL−1 (where TCID50 is the 50 % tissue culture infective dose). The detection limit is 210 TCID50⋅mL−1 (at an S/N of 3), and the correlation coefficient (R) is 0.9964. The electrochemical immunoassay showed good selectivity, stability and reproducibility.

Schematic illustration of the stepwise immunosensor fabrication process

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 21075078, 21105056) and the Natural Science Foundation of Shandong province, China (No. ZR2010BM005, ZR2011BQ001).

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Authors and Affiliations

  1. College of Chemistry and Material Science, Shandong Agricultural University, Taian, Shandong, 271018, People’s Republic of China

    Kun Shang, Jing Dong & Shiyun Ai

  2. College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong, 271018, People’s Republic of China

    Zhenzhen Wang & Ziqiang Cheng

Authors
  1. Zhenzhen Wang
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  2. Kun Shang
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  3. Jing Dong
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  4. Ziqiang Cheng
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  5. Shiyun Ai
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Corresponding authors

Correspondence to Ziqiang Cheng or Shiyun Ai.

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Zhenzhen Wang and Kun Shang contributed equally to this work.

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Wang, Z., Shang, K., Dong, J. et al. Electrochemical immunoassay for subgroup J of avian leukosis viruses using a glassy carbon electrode modified with a film of poly (3-thiophene boronic acid), gold nanoparticles, graphene and immobilized antibody. Microchim Acta 179, 227–234 (2012). https://doi.org/10.1007/s00604-012-0874-6

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  • DOI: https://doi.org/10.1007/s00604-012-0874-6

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