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Comparison of three different configurations of dual ultramicroelectrodes for the decomposition of S-Nitroso-L-glutathione and the direct detection of nitric oxide

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Abstract

We report on the characterization and use of three configurations of dual gold ultramicroelectrodes (UMEs), namely a ring-disc electrode, a disc-disc electrode, and band-band electrode. These can be used for the direct determination of nitric oxide that is released during the copper-catalyzed decomposition of S-nitroso-L-glutathione (GSNO). The dual UMEs were electrochemically characterized by using ferrocenemethanol as a soluble redox mediator during chronoamperometric measurements. The data are similar to those reported in the literature for various configurations and can be used to characterize the ability of copper ions to reach the vicinity of the NO sensor. One UME was electrochemically modified with layers of a poly(eugenol)/poly(phenol) composite to act as an NO sensor. The second UME was electrochemically coated with a copper layer that serves as a source for Cu(II) that is needed for the in-situ decomposition of GSNO. The mediated decomposition of GSNO is accomplished in presence of ascorbate that acts as reducing agent for Cu(II). The NO released from GSNO is detected at the same potential as applied to form the Cu(II)-based catalyst (+0.8 V vs Ag/AgCl).

We report on the characterization and use of three configurations of dual gold ultramicroelectrodes namely a ring-disc electrode, a disc-disc electrode, and band-band electrode. These can be used for the direct determination of nitric oxide that is released during the copper-catalyzed decomposition of S-nitroso-L-glutathione.

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Acknowledgements

Financial for this research was supported by “Agence Nationale de la Recherche” (ANR France) in the framework of the project MECANO ANR-08-PCVI-0018. AN is thankful to Université de Douala (Cameroun) for offering him the opportunity to travel to Paris for this work.

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Authors and Affiliations

  1. Laboratoire de Chimie Analytique, Département de Chimie, Faculté des Sciences, Université de Douala, B.P. 24157, 24167, Douala, Cameroun

    Achille Nassi

  2. Chimie ParisTech, Ecole Nationale Supérieure de Chimie de Paris, Unité de Pharmacologie Chimique et Génétique et Imagerie, 75005, Paris, France

    Achille Nassi, Loan To Thi Kim, Sophie Griveau & Fethi Bedioui

  3. CNRS, Unité de Pharmacologie Chimique et Génétique et Imagerie UMR 8151, 75005, Paris, France

    Loan To Thi Kim, Sophie Griveau & Fethi Bedioui

  4. Université Paris Descartes, Unité de Pharmacologie Chimique et Génétique et Imagerie, 75006, Paris, France

    Loan To Thi Kim, Sophie Griveau & Fethi Bedioui

  5. INSERM, Unité de Pharmacologie Chimique et Génétique et Imagerie (N° 1022), 75006, Paris, France

    Loan To Thi Kim, Sophie Griveau & Fethi Bedioui

  6. CNRS 8029 SATIE, Ecole Normale Supérieure de Cachan-Bretagne, 35170, Bruz, France

    Aurélie Girard, Laurent Griscom & Florence Razan

  7. CNRS 8640, Ecole Normale Supérieure, 75005, Paris, France

    Laurent Thouin

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  1. Achille Nassi
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Correspondence to Fethi Bedioui.

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Nassi, A., To Thi Kim, L., Girard, A. et al. Comparison of three different configurations of dual ultramicroelectrodes for the decomposition of S-Nitroso-L-glutathione and the direct detection of nitric oxide. Microchim Acta 179, 337–343 (2012). https://doi.org/10.1007/s00604-012-0860-z

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