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Determination of gabapentin in human plasma by capillary electrophoresis-laser induced fluorescence detection with and without solid-phase extraction

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Abstract

We have developed two methods for the quantitation of gabapentin in human plasma. They are based on capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) with and without solid-phase extraction (SPE) and the derivatizing reagent 5-(4,6-dichlorotriazinyl)amino fluoresencin. The conditions for derivatization, separation and extraction were investigated in detail, and the optimal labeling conditions include a temperature of 40 °C, a reaction time of 30 min, and the use of a borate buffer of pH 9.0 as the reaction medium. A borate buffer of pH 9.2 served as a background electrolyte for CE separations. The CE-LIF and SPE-CE-LIF methods have linear ranges of 5–200 nmol L−1 and 0.2–10 nmol L−1, respectively, and the limits of detection are 0.5 and 0.02 nmol L−1, respectively. The SPE-CE-LIF method was successfully applied to the determination of gabapentin in blood plasma samples.

Two methods have been developed for the quantization of gabapentin (GBP) in human plasma. They are based on capillary electrophoresis-laser induced fluorescence detection (CE-LIF) with and without solid-phase extraction (SPE) and the derivatizing reagent 5-(4,6-dichlorotriazinyl)aminofluorescein.

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Acknowledgement

This work was supported by “the fundamental research funds for the central universities” (NO. 11609702).

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Authors and Affiliations

  1. Department of Chemistry, College of Life Science and Technology, Jinan University, Guangzhou, 510632, China

    Liwei Cao, Siliu Liang, Xiaofang Tan & Jianxin Meng

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  1. Liwei Cao
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  2. Siliu Liang
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  3. Xiaofang Tan
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Correspondence to Liwei Cao.

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Cao, L., Liang, S., Tan, X. et al. Determination of gabapentin in human plasma by capillary electrophoresis-laser induced fluorescence detection with and without solid-phase extraction. Microchim Acta 178, 285–292 (2012). https://doi.org/10.1007/s00604-012-0835-0

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  • DOI: https://doi.org/10.1007/s00604-012-0835-0

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