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Direct electrochemistry of hemoglobin on an ionic liquid carbon electrode modified with zinc tungstate nanorods

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Abstract

We have constructed a new electrochemical biosensor by immobilization of hemoglobin (Hb) and ZnWO4 nanorods in a thin film of chitosan (CTS) on the surface of carbon ionic liquid electrode. UV–vis and FT-IR spectra reveal that Hb remains in its native conformation in the film. The modified electrode was characterized by scanning electron microscopy, electrochemical impedance spectroscopy and cyclic voltammetry. A pair of well-defined redox peaks appears which indicates direct electron transfer from the electrode. The presence of CTS also warrants biocompatibility. The electron transfer coefficient and the apparent heterogeneous electron transfer rate constant were calculated to be 0.35 and 0.757 s−1, respectively. The modified electrode displays good electrocatalytic activity for the reduction of trichloroacetic acid with the detection limit of 0.613 mmol L−1 (3σ). The results extend the protein electrochemistry based on the use of ZnWO4 nanorods.

A ZnWO4 nanorods and hemoglobin nanocomposite material modified carbon ionic liquid electrode was used as the platform for the construction of an electrochemical hemoglobin biosensor.

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Acknowledgments

We are grateful to the financial support of the Natural Science Foundation of Jiangxi Province (2009GZC0031) and the S&T plan projects of Jiangxi Provincial Education Department (GJJ10590).

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Authors and Affiliations

  1. Jiangxi Key Laboratory of Surface Engineering, Jiangxi Science and Technology Normal University, Nanchang, 330013, People’s Republic of China

    Chengxiang Ruan, Jin Lou, Weimin Gao & Yunsheng Xiao

  2. College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, People’s Republic of China

    Zhaolan Sun, Jun Liu & Wei Sun

Authors
  1. Chengxiang Ruan
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  2. Zhaolan Sun
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  3. Jun Liu
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  4. Jin Lou
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  5. Weimin Gao
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  6. Wei Sun
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  7. Yunsheng Xiao
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Correspondence to Chengxiang Ruan or Wei Sun.

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Ruan, C., Sun, Z., Liu, J. et al. Direct electrochemistry of hemoglobin on an ionic liquid carbon electrode modified with zinc tungstate nanorods. Microchim Acta 177, 457–463 (2012). https://doi.org/10.1007/s00604-012-0796-3

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  • DOI: https://doi.org/10.1007/s00604-012-0796-3

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