Abstract
We have fabricated an interdigitated double-coil microelectrode chip for the determination of traces of phosphate by making use of a MEMS technique and enzyme immobilization technology. The chip is composed of two 3-dimensional strip microelectrodes which form a double coil microelectrode configuration with steep sidewalls and high aspect ratio. This novel configuration results in a high current response during amplification by redox cycling. The enzyme pyruvate oxidase was immobilized on the chip using gold nanoparticles as a support. Phosphate can be determined by using this chip with good sensitivity and linearity and in concentrations ranging from 0.5 μM to 7 μM.
References
Seena V, Rajorya A, Pant P, Mukherji S, Ramgopal Rao V (2009) Polymer microcantilever biochemical sensors with integrated polymer composites for electrical detection. Solid State Sci 11:1606
Forleo A, Francioso L, Capone S, Casino F, Siciliano P, Tan OK, Hui H (2011) Fabrication at wafer level of miniaturized gas sensors based on SnO2 nanorods deposited by PECVD and gas sensing characteristics. Sens Actuators B 154:283
Akyilmaz E, Turemis M (2010) An inhibition type alkaline phosphatase biosensor for amperometric determination of caffeine. Electrochim Acta 55:5195
Kudo H, Suzuki Y, Gessei T, Takahashi D, Arakawa T, Mitsubayashi K (2010) Biochemical gas sensor (bio-sniffer) for ultrahigh-sensitive gaseous formaldehyde monitoring. Biosens Bioelectron 26:854
Zougagh M, Ríos Á (2009) Micro-electromechanical sensors in the analytical field. Analyst 134:1274
Chang J, Wei GT, Zen JM (2011) Screen-printed ionic liquid/preanodized carbon electrode: effective detection of dopamine in the presence of high concentration of ascorbic acid. Electrochem Commun 13:174
Strand N, Bhushan A, Schivo M, Kenyon NJ, Davis CE (2010) Chemically polymerized polypyrrole for on-chip concentration of volatile breath metabolites. Sens Actuators B 143:516
Park J, Pak YK, Pak JJ (2010) A microfabricated reservoir-type oxygen sensor for measuring the real-time cellular oxygen consumption rate at various conditions. Sens Actuators B 147:263
Chen YP, Zhao Y, Chu J, Liu SY, Li WW, Liu G, Tian YC, Xiong Y, Yu HQ (2010) Fabrication and characterization of an innovative integrated solid-state microelectrode. Electrochim Acta 55:5984
Guiseppi-Elie A, Abdur Rahman AR, Shukla NK (2010) SAM-modified microdisc electrode arrays (MDEAs) with functionalized carbon nanotubes. Electrochim Acta 55:4247
Shan C, Yang H, Song J, Han D, Ivaska A, Niu L (2009) Direct electrochemistry of glucose oxidase and biosensing for glucose based on graphene. Anal Chem 81:2378
Cui K, Song Y, Yao Y, Huang Z, Wang L (2008) A novel hydrogen peroxide sensor based on Ag nanoparticles electrodeposited on DNA-networks modified glassy carbon electrode. Electrochem Commun 10:663
Salimi A, Noorbakhsh A (2011) Layer by layer assembly of glucose oxidase and thiourea onto glassy carbon electrode: fabrication of glucose biosensor. Electrochim Acta 56:6097
Crew A, Lonsdale D, Byrd N, Pittson R, Hart JP (2011) A screen-printed, amperometric biosensor array incorporated into a novel automated system for the simultaneous determination of organophosphate pesticides. Biosens Bioelectron 26:2847
Gopalan AI, Lee KP, Ragupathy D, Lee SH, Lee JW (2009) An electrochemical glucose biosensor exploiting a polyaniline grafted multiwalled carbon nanotube/perfluorosulfonate ionomer-silica nanocomposite. Biomaterials 30:5999
Montenegro MI, Arlete Queirós M, Daschbach JL (1991) Microelectrodes: theory and applications, NATO Science Series E 197, ISBN: 0792312295
Lu Y, Xu H, Wang J, Zhong L (2011) Predicting size effect on diffusion-limited current density of oxygen reduction by copper wire. Chin J Oceanol Limnol 29:75
Balasubramanian K (2010) Challenges in the use of 1D nanostructures for on-chip biosensing and diagnostics: a review. Biosens Bioelectron 26:1195
Gardner RD, Zhou A, Zufelt NA (2009) Development of a microelectrode array sensing platform for combination electrochemical and spectrochemical aqueous ion testing. Sens Actuators B 136:177
Daniel D, Gutz IGR (2005) Microfluidic cells with interdigitated array gold electrodes: fabrication and electrochemical characterization. Talanta 68:429
Honda N, Inaba M, Katagiri T, Shoji S, Sato H, Homma T, Osaka T, Saito M, Mizuno J, Wada Y (2005) High efficiency electrochemical immuno sensors using 3D comb electrodes. Biosens Bioelectron 20:2306
Montelius L, Tegenfeld JO, Ling TGI (1995) Fabrication and characterization of a nanosensor for admittance spectroscopy of biomolecules. J Vac Sci Tech A 13:1755
Ji X, Jin B, Ren J, Jin J, Nakamura T (2005) Electrochemical characterization of poly(acrylamide) membrane coated on a gold disk electrode in acetonitrile. J Electroanal Chem 579:25
Richter EM, Munoz RAA, Bertotti M, Angnes L (2007) Heat-transference of toner masks onto conductive substrates: a rapid and easy way to produce microelectrode ensembles. Electrochem Commun 9:1091
Heo JI, Shim DS, Turon Teixidor G, Oh S, Madou MJ, Shin H (2011) Carbon interdigitated array nanoelectrodes for electrochemical applications. J Electrochem Soc 158:J76
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This work is supported by the National Basic Research Program of China (973 Program) (Project Number 2009CB320300) and the Natural Science Foundation of China (60971070).
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Xue, Q., Bian, C., Tong, J. et al. Fabrication of a 3D interdigitated double-coil microelectrode chip by MEMS technique. Microchim Acta 177, 491–496 (2012). https://doi.org/10.1007/s00604-012-0770-0
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DOI: https://doi.org/10.1007/s00604-012-0770-0