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Sensor for hydrogen peroxide using a hemoglobin-modified glassy carbon electrode prepared by enhanced loading of silver nanoparticle onto carbon nanospheres via spontaneous polymerization of dopamine

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Abstract

We have developed a simple and efficient method for the enhanced loading of silver nanoparticles onto carbon nanospheres, and how this method can be used to design an electrochemical sensor for hydrogen peroxide (HP). A glassy carbon electrode was modified with hemoglobin, carbon nanospheres, and by enhanced loading of silver nanoparticles onto the carbon nanospheres via spontaneous polymerization of dopamine. The hemoglobin exhibits a remarkable electrocatalytic activity for the reduction of HP. The electrochemical response to HP is linear range in the 1.0–147.0 μM concentration range, with a detection limit of 0.3 μM at a signal-to-noise ratio of 3.

A simple and efficient method has developed for enhanced loading of silver nanoparticles onto carbon nanospheres via polydopamine (AgNP-Pdop@CNPs). The direct chemistry of hemoglobin has been achieved at the AgNP-Pdop@CNPs modified glassy carbon electrode and the modified electrode exhibits a remarkable electrocatalytic activity for the reduction of hydrogen peroxide. The electrochemical response to H2O2 shows a linear range of 1.0–147.0 μM with a calculated detection limit of 0.3 μM at a signal-to-noise ratio of 3

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Acknowledgements

Y. Wang and M. Li are grateful for financial support from the Natural Science Foundation of China (Grant Nos. 20801001 and 21075001) and Anhui Provincial Natural Science Foundation (Grant No. 11040606 M46). F. Gao thanks the Natural Science Foundation of China (Grant Nos. 21055001 and 21175002) for financial support. X. Lin thanks the Special Foundation for Young Scientists of Hefei Institutes of Physical Science (Chinese Academy of Sciences) for financial support.

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Authors and Affiliations

  1. Key Laboratory of Chemo-Biosensing, Anhui Province; College of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241000, People’s Republic of China

    Yinling Wang, Min Tang, Feng Gao & Maoguo Li

  2. The State Key Laboratory of Transducer Technology, Hefei Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, 230031, People’s Republic of China

    Min Tang & Xinhua Lin

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  1. Yinling Wang
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  2. Min Tang
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  3. Xinhua Lin
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  4. Feng Gao
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  5. Maoguo Li
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Correspondence to Maoguo Li.

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Wang, Y., Tang, M., Lin, X. et al. Sensor for hydrogen peroxide using a hemoglobin-modified glassy carbon electrode prepared by enhanced loading of silver nanoparticle onto carbon nanospheres via spontaneous polymerization of dopamine. Microchim Acta 176, 405–410 (2012). https://doi.org/10.1007/s00604-011-0736-7

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  • DOI: https://doi.org/10.1007/s00604-011-0736-7

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