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Rapid separation of four probiotic bacteria in mixed samples using microchip electrophoresis with laser-induced fluorescence detection

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Abstract

Microchip electrophoresis (MCE) coupled to laser-induced fluorescence detection was applied to the rapid separation of Bifidobacterium, Lactobacillus casei, Lactobacillus acidophilus, and Enterococccus faecalis. All bacteria were quickly separated within 150 s using a running buffer of pH 8.5 containing Tris, borate, EDTA, and poly(ethylene oxide). The latter was crucial to reduce the bacterial adsorption on the walls of the microchannels. The pH of 8.5 warrants that bacteria carry a negative charge at their surface and thus display good electrophoretic performance. The method was used to analyze medical samples containing these probiotics, and the results showed that the identification and detection of bacteria by MCE is advantageous in terms of sample consumption, waste production, time of analysis, and instrumental effort.

Four probiotic bacteria was rapidly separated by microchip electrophoresis with laser-induced fluorescence detection

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Acknowledgements

This work was financially supported by the program for New Century Excellent Talents in University. We are grateful to Shanghai Spectrum Company for the assistance with the fabrication of the LIF detection.

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Authors and Affiliations

  1. Department of Chemistry, East China Normal University, Shanghai, 200062, People’s Republic of China

    Shuang Cheng, Zhifang Wang, Shuli Ge, Huan Wang, Pingang He, Yuzhi Fang & Qingjiang Wang

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  1. Shuang Cheng
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  2. Zhifang Wang
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  3. Shuli Ge
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  4. Huan Wang
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  5. Pingang He
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  6. Yuzhi Fang
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  7. Qingjiang Wang
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Correspondence to Yuzhi Fang or Qingjiang Wang.

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Cheng, S., Wang, Z., Ge, S. et al. Rapid separation of four probiotic bacteria in mixed samples using microchip electrophoresis with laser-induced fluorescence detection. Microchim Acta 176, 295–301 (2012). https://doi.org/10.1007/s00604-011-0728-7

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  • DOI: https://doi.org/10.1007/s00604-011-0728-7

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