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Rapid detection of Staphylococcus aureus via a sensitive DNA hybridization assay based on a long-lifetime luminescent europium marker

Abstract

A two-probe tandem nucleic acid hybridization assay for detection of Staphylococcus aureus is presented. It is based on a europium(III) complex as a marker that has a long fluorescence lifetime, high quantum yield and can be easily conjugated to an oligonucleotide signaling probe. The amino-modified capture probe was associated with the signaling probe to form a two-probe tandem DNA pattern that is complementary to the target DNA. The method was optimized in terms of hybridization temperature, hybridization time and washing time. This resulted in good specificity and sensitivity when detecting such bacteria in food samples.

A europium complex as a long fluorescent lifetime marker was conjugated to an oligonucleotide. The amino-modified capture probe was associated with the signaling probe to form a two-probe tandem DNA pattern that is complementary to the target DNA. The results are shown that this method has good specificity and sensitivity.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (20977026, 51039001), the National 863 High Technology Research Foundation of China (2006AA06Z407), the Research Fund for the Doctoral Program of Higher Education of China (20090161110009), and the Equipment Foundation of Hunan University.

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Correspondence to Cheng-Gang Niu.

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Ruan, M., Niu, CG., Zeng, GM. et al. Rapid detection of Staphylococcus aureus via a sensitive DNA hybridization assay based on a long-lifetime luminescent europium marker. Microchim Acta 175, 105 (2011). https://doi.org/10.1007/s00604-011-0654-8

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  • DOI: https://doi.org/10.1007/s00604-011-0654-8

Keywords

  • Staphylococcus aureus
  • Capture probe
  • Signaling probe
  • Long lifetime biomarker
  • DNA hybridization
  • Pathogenic microorganisms monitoring