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A biopolymer-based carbon nanotube interface integrated with a redox shuttle and a D-sorbitol dehydrogenase for robust monitoring of D-sorbitol

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Abstract

We describe the preparation and characterization of a glassy carbon electrode modified with a bionanocomposite consisting of a hyaluronic acid, dispersed carbon nanotubes, and electrostatically bound toluidine blue. The electrode was used to detect NADH in the batch and flow-injection mode of operation. The electrode was further modified by immobilizing sorbitol dehydrogenase to result in biosensor for D-sorbitol that displays good operational stability, a sensitivity of 10.6 μA mM−1 cm−2, a response time of 16 s, and detection limit in the low micromolar range. The biosensor was successfully applied to off-line monitoring of D-sorbitol during its bioconversion into L-sorbose (a precursor in the synthesis of vitamin C) by Gluconobacter oxydans. The sample assay precision is 2.5% (an average RSD) and the throughput is 65 h−1 if operated in the flow-injection mode. The validation of this biosensor against a reference HPLC method resulted in a slope of correlation of 1.021 ± 0.001 (R 2 = 0.99997).

Immobilisation of D-sorbitol dehydrogenase between two biopolymers on carbon nanotube layer provides stable and robust D-sorbitol biosensing with a mediator being electrostatically bound within the matrix. The biosensor was succesfully applied in analysis of fermentation samples with througput of assays of 65 h−1 in flow system.

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Acknowledgements

The financial supports from projects VEGA 1/0066/09 and 1/0335/10; COST CM8T D43 and SAV-FM-EHP-2008-04-04 are acknowledged. This contribution/publication is the result of the project implementation: Centre for materials, layers and systems for applications and chemical processes under extreme conditions supported by the Research & Development Operational Program funded by the ERDF.

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Authors and Affiliations

  1. Department of Glycobiotechnology, Center for Glycomics, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-845 38, Bratislava, Slovakia

    Jana Šefčovičová, Jaroslav Filip, Peter Gemeiner, Marek Bučko, Peter Magdolen & Jan Tkac

  2. Institute of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, SK-812 37, Bratislava, Slovakia

    Peter Tomčík

  3. Department of Chemistry, Faculty of Education, The Catholic University in Ružomberok, Hrabovská cesta 1, SK-034 01, Ružomberok, Slovakia

    Peter Tomčík

Authors
  1. Jana Šefčovičová
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  2. Jaroslav Filip
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  3. Peter Tomčík
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  4. Peter Gemeiner
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  5. Marek Bučko
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  7. Jan Tkac
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Correspondence to Jan Tkac.

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Šefčovičová, J., Filip, J., Tomčík, P. et al. A biopolymer-based carbon nanotube interface integrated with a redox shuttle and a D-sorbitol dehydrogenase for robust monitoring of D-sorbitol. Microchim Acta 175, 21–30 (2011). https://doi.org/10.1007/s00604-011-0641-0

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