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Chitosan matrices modified with carbon nanotubes for use in mediated microbial biosensing

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Abstract

We describe a microbial sensor based on Pseudomonas fluorescens cells that was prepared by modifying graphite electrodes with chitosan and carbon nanotubes. Chronoamperometry was performed at +0.3 V in the presence of hexacyanoferrate as a mediator and revealed a good response to glucose which is linear in the 1.0 to 5.0 mM concentration range. Linearity was defined by the equation of y = 102.120x−13.279 (R 2 = 0.998) (y shows current density as nA.cm−2 and x shows glucose concentration in mM). The effect of the CNTs on the response was compared to that of electrodes made without CNTs.

A mediated microbial sensor that was prepared by modifying graphite electrodes with chitosan and carbon nanotube and Pseudomonas fluorescens cells has been described. As well as some parameters (pH, mediator and cell amount etc), the effect of CNTs on the response was compared to that of electrodes made without CNTs.

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Acknowledgement

Ege University Research Foundation (project number: 2010 FEN 001) is acknowledged for the financial support.

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  1. Faculty of Science, Biochemistry Department, Ege University, 35100, Bornova-Izmir, Turkey

    Dilek Odaci Demirkol & Suna Timur

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  1. Dilek Odaci Demirkol
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  2. Suna Timur
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Correspondence to Dilek Odaci Demirkol or Suna Timur.

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Demirkol, D.O., Timur, S. Chitosan matrices modified with carbon nanotubes for use in mediated microbial biosensing. Microchim Acta 173, 537–542 (2011). https://doi.org/10.1007/s00604-011-0596-1

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  • DOI: https://doi.org/10.1007/s00604-011-0596-1

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