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Glassy carbon electrode modified with poly-Neutral Red for photoelectrocatalytic oxidation of NADH

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Abstract

A new approach is described for the photoelectrocatalytic oxidation of Reduced ß-Nicotinamide Adenine Dinucleotide (NADH). It is based on a glassy carbon electrode (GCE) modified with a film of poly-Neutral Red (poly-NR) that is obtained by electropolymerization. Electrochemical measurements revealed that the modified electrode displays electrocatalytic and photo-electrocatalytic activity towards oxidation of NADH. If irradiated with a 250-W halogen lamp, the electrode yields a strongly increased electrocatalytic current compared to the current without irradiation. Amperometric and photo-amperometric detection of NADH was performed at +150 mV vs. Ag/AgCl/KClsat and the currents obtained are linearly related to the concentration of NADH. Linear calibration plots are obtained in the concentration range from 1.0 μM to 1.0 mM for both methods. However, the slope of the current-NADH concentration curve of the photo-electrocatalytic procedure was 2-times better than that obtained without irradiation.

A poly-Neutral Red modified glassy carbon electrode (poly-NR/GCE) was prepared by electropolymerization process. This modified electrode displays electrocatalytic and also photoelectrocatalytic activity towards oxidation of NADH. Compared with electrocatalytic oxidation of NADH, the current response was increased about 2.0 times in the photoelectrocatalytic oxidation process.

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Acknowledgments

The authors acknowledge the Scientific and Technological Research Council of Turkey TUBITAK (Project No. 107T572) and The National Authority for Scientific Research of Romania ANCS (Project no. 75CB/18.07.2008) for financial support. Y. Dilgin would like to thank Çanakkale Onsekiz Mart University Scientific Research Project Commission Fund for partially supporting of this work with a project (COMU BAP 2009/15).

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Authors and Affiliations

  1. Biga Vocational College, Çanakkale Onsekiz Mart University, TR-17200, Biga Çanakkale, Turkey

    Didem Giray Dilgin

  2. Department of Chemistry, Ege University, Science Faculty, 35100, Bornova/İzmir, Turkey

    Didem Giray Dilgin, H. İsmet Gökçel & Zekerya Dursun

  3. Department of Environmental Physics, Chemistry and Engineering, Babes-Bolyai University, 30 Fantanele St., 400294, Cluj-Napoca, Romania

    Delia Gligor

  4. Department of Chemistry, Science & Art Faculty, Çanakkale Onsekiz Mart University, 17100, Çanakkale, Turkey

    Yusuf Dilgin

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  1. Didem Giray Dilgin
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Dilgin, D.G., Gligor, D., Gökçel, H.İ. et al. Glassy carbon electrode modified with poly-Neutral Red for photoelectrocatalytic oxidation of NADH. Microchim Acta 173, 469–476 (2011). https://doi.org/10.1007/s00604-011-0582-7

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