Abstract
We describe the synthesis of spherical poly(vinyl butyral) (PVB) nanobeads by controlled precipitation via addition of non-solvent. Effects of various reaction parameters on nanoparticle size were investigated by dynamic light scattering and electron microscopy. The ability to incorporate dopant molecules was studied using a fluorescent perylene derivative as a model additive, and the dye-doped nanoparticles were investigated by confocal microscopy. In an optimized experimental protocol, PVB nanoparticles were obtained that were efficiently taken up by human cancer cells devoid of coating. The novel nanospheres are economic, easy to prepare and capable of incorporating additives. Lacking cytotoxicity in vitro, PVB nanobeads are attractive with respect to various potential applications such as optical imaging and particle tracking, diagnostics, and drug delivery.
The synthesis and characterization of polyvinyl butyral nanoparticles is described. The beads were doped with a dye and used for intracellular fluorescence imaging. The nanospheres are efficiently taken up without coating and do not display in vitro cytotoxicity on human cancer cells. They are therefore attractive for various applications.
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Acknowledgements
We thank Heiko I. Siegmund and Dr. Josef Schröder (Institute of Pathology, University Hospital Regensburg) for transmission electron microscopy and Dr. Michael Frank (Kuraray Specialities Europe GmbH) for donation of polyvinyl butyral Mowital LPB 16 H. D.P. and U.B. gratefully acknowledge the hospitality in the Institute of Physical Chemistry (chair: Prof. Dr. B. Dick). This work was partially supported by the graduate college GRK 640 of the Deutsche Forschungsgemeinschaft (DFG).
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Posavec, D., Dorsch, A., Bogner, U. et al. Polyvinyl butyral nanobeads: preparation, characterization, biocompatibility and cancer cell uptake. Microchim Acta 173, 391–399 (2011). https://doi.org/10.1007/s00604-011-0573-8
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DOI: https://doi.org/10.1007/s00604-011-0573-8