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Voltammetric sensing of guanine and adenine using a glassy carbon electrode modified with a tetraoxocalix[2]arene[2]triazine Langmuir-Blodgett film

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Abstract

We have prepared a new voltammetric sensor for guanine and adenine. It is based on a glassy carbon electrode modified with a Langmuir-Blodgett film made from tetraoxocalix[2]arene[2]triazine. The direct electro-oxidation of adenine and guanine was investigated and the results indicat that in contrast to a bare glassy carbon electrode both guanine and adenine cause an increase in the oxidation peak currents along with a negative shift of the oxidation potentials. The electrode enables the simultaneous determination of guanine and adenine using square wave voltammetry. Analysis of acid denatured calf thymus DNA was carried out and the value of (G + C)/(A + T) was correctly found to be 0.75.

Both guanine and adenine showed the increase of the oxidation peak currents on LBTOCT-GCE in contrast to that on the bare glassy carbon electrode (GCE).

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Acknowledgements

The authors gratefully acknowledge the financial support of the National Natural Science Foundation of China (No. 20775073, 20875083).

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Authors and Affiliations

  1. Department of Chemistry, Zhengzhou University, Zhengzhou, 450052, The People’s Republic of China

    Lina Zou, Yamin Li & Baoxian Ye

  2. Department of Chemistry, Zhengzhou University, Zhengzhou, Henan, 450001, People’s Republic of China

    Baoxian Ye

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  1. Lina Zou
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  2. Yamin Li
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Correspondence to Baoxian Ye.

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Zou, L., Li, Y. & Ye, B. Voltammetric sensing of guanine and adenine using a glassy carbon electrode modified with a tetraoxocalix[2]arene[2]triazine Langmuir-Blodgett film. Microchim Acta 173, 285–291 (2011). https://doi.org/10.1007/s00604-011-0563-x

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