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Sensitive DNA-hybridization biosensors based on gold nanoparticles for testing DNA damage by Cd(II) ions

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Abstract

A DNA biosensor was constructed by immobilizing a 20-mer oligonucleotide probe and hybridizing it with its complementary oligomer on the surface of a glassy carbon electrode modified with gold nanoparticles. The properties of the biosensor and its capability of recognizing its complementary sequence were studied by electrochemical impedance spectroscopy. The oxidative stress caused by cadmium ions can be monitored by differential pulse voltammetry using the cobalt(III)tris(1,10-phenanthroline) complex and methylene blue as electrochemical indicators. The biosensor is capable of indicating damage caused by Cd(II) ions in pH 6.0 solution. The results showed that the biosensor can be used for rapid screening for DNA damage.

DPV of DNA biosensors before (a, c) and after hybridization (b, d) at 1.0 ×10–7 mol·L-1target DNA concentration, (a) probe DNA/Au/GCE and (b) dsDNA/Au/GCE (c) probe DNA/GCE, (d) dsDNA/GCE

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Acknowledgments

We thank the National Natural Science Foundation of China (Grant No. 20775002) for financial support. The work was supported by the Program for Innovative Research Team in Anhui Normal University.

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Authors and Affiliations

  1. Anhui Key Laboratory of Chemo-Biosening, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241000, People’s Republic of China

    Qianru Zhang, Panpan Dai & Zhousheng Yang

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  1. Qianru Zhang
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  2. Panpan Dai
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  3. Zhousheng Yang
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Correspondence to Zhousheng Yang.

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Fig. S1

Nyquist diagrams recorded at the probe DNA/Au/GCE (a) and after hybridization reaction with different concentrations of sequence- specific DNA of PAT gene: (b) 1.0 × 10−12 mol.L−1, (c) 1.0 × 10−11 mol.L−1, (d) 1.0 × 10−10 mol.L−1, (e) 1.0 × 10−9 mol.L−1, (f) 1.0 × 10−8 mol.L−1, (g) 1.0 × 10−7 mol.L−1 and (h) 1.0 × 10−6 mol.L−1. (DOC 554 kb)

Fig. S2

Nyquist diagrams recorded of dsDNA/nano- Au/GCE in 5.0 mmol.L−1 pH 6.0 Tris–HCl buffer solution after damaged by 0.02 mmol.L−1 Cd2+ for different time: 0 min, 10 min, 20 min, 30 min,50 min (a→e). (DOC 475 kb)

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Zhang, Q., Dai, P. & Yang, Z. Sensitive DNA-hybridization biosensors based on gold nanoparticles for testing DNA damage by Cd(II) ions. Microchim Acta 173, 347–352 (2011). https://doi.org/10.1007/s00604-011-0558-7

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  • DOI: https://doi.org/10.1007/s00604-011-0558-7

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