Skip to main content
SpringerLink
Log in

Simple construction of an enzymatic glucose biosensor based on a nanocomposite film prepared in one step from iron oxide, gold nanoparticles, and chitosan

  • Original Paper
  • Published:
Microchimica Acta Aims and scope Submit manuscript

Abstract

The one-step synthesis is reported of a nanofilm composed of iron oxide and gold nanoparticles in a chitosan matrix that can act as a novel matrix for the immobilization of glucose oxidase (GOx) to fabricate a glucose biosensor. The use for the composite film strongly increased the effective electrode surface for loading of GOx. The size and shape of the iron oxide nanoparticles were examined by transmission electron micrograph. Direct electron transfer and electrocatalysis by GOx was investigated via cyclic voltammetry and chronoamperometry. Under optimized conditions, the biosensor has a response time of 6 s and a linear response in the range between 3 μM and 0.57 mM of glucose, with a detection limit of 1.2 μM at a signal-to-noise ratio of 3. This novel and disposable mediatorless glucose biosensor may form the basis for a future mass-produced glucose biosensor.

In this paper, based on the direct electrochemistry of redox enzyme, we try to integrate the excellent properties of iron oxide-gold nanoparticle-chitosan composite film with the advantages of one-step electrodeposition to fabricate a sensitive and stable glucose biosensor.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Zhang XJ, Ju HX, Wang J (2008) Electrochemical sensors, biosensors and their biomedical applications. Elsevier, New York

    Google Scholar 

  2. Heller A (1990) The role of oxygen in photooxidation of organic molecules on semiconductor particles. Acc Chem Res 23:128

    Article  CAS  Google Scholar 

  3. Willner I, Katz E (2000) Integration of layered redox-proteins and conductive supports for bioelectronic applications. Angew Chem Int Ed 39:1180

    Article  Google Scholar 

  4. Lojou É, Bianco P (2000) Membrane electrodes can modulate the electrochemical response of redox proteins-direct electrochemistry of cytochrome c. J Electroanal Chem 485:71

    Article  CAS  Google Scholar 

  5. Gu H, Yu A, Chen H (2001) Direct electron transfer and characterization of hemoglobin immobilized on a gold colloid-cysteamine-modified gold electrode. J Electroanal Chem 516:119

    Article  CAS  Google Scholar 

  6. Wang Q, Lu G, Yang B (2004) Myoglobin/sol-gel film modified electrode: direct electrochemistry and electrochemical catalysis. Langmuir 20:1342

    Article  Google Scholar 

  7. Yang J, Zhang RY, Xu Y, He PG, Fang YZ (2008) Direct electrochemistry study of GOx on Pt nanoparticle-modified aligned carbon nanotubes electrode by the assistance of CS-CdS and its biosensoring for glucose. Electrochem Commun 10:1889

    Article  Google Scholar 

  8. Liu Q, Lu XB, Li J, Yao X, Li JH (2007) Direct electrochemistry of GOx and electrochemical biosensing of glucose on quantum dots/carbon nanotubes electrodes. Biosens Bioelectron 22:3203

    Article  CAS  Google Scholar 

  9. Salimi A, Roushani M (2005) Nonenzymatic glucose detection free of ascorbic acid interference using nickel powder and nafion sol-gel dispersed renewable carbon ceramic electrode. Electrochem Commun 7:879

    Article  CAS  Google Scholar 

  10. Wang Y, Wei WZ, Liu XY, Zeng XD (2009) GNPs/CNTs/CS-based glucose biosensor prepared by a layer-by-layer technique. Mater Sci Eng C 29:50

    Article  Google Scholar 

  11. Lin JH, He CY, Zhao Y, Zhang SS (2009) One-step synthesis of silver nanoparticles/CNTs/CS film and its application in glucose biosensor. Sens Actuators B 137:768

    Article  Google Scholar 

  12. Xue MH, Xu Q, Zhou M, Zhu JJ (2006) In situ immobilization of GOx in CS-nanoparticle hybrid film on Prussian Blue modified for high sensitivity glucose detection. Electrochem Commun 8:1468

    Article  CAS  Google Scholar 

  13. Luo XL, Xu JJ, Du Y, Chen HY (2004) A glucose biosensor based on CS-GOx-GNPs biocomposite formed by one-step electrodeposition. Anal Biochem 334:284

    Article  CAS  Google Scholar 

  14. Wang KQ, Yang H, Zhu L, Liao JH, Lu TH, Xing W, Xing SY, Lv Q (2009) Direct electrochemistry and electrocatalysis of GOx immobilized on glassy carbon electrode modified by Nafion and ordered mesoporous silica-SBA-15. J Mol Catal B Enzym 58:194

    Article  CAS  Google Scholar 

  15. Kim DK, Mikhaylova M, Zhang Y, Muhammed M (2003) Protective coating of superparamagnetic iron oxide nanoparticles. Chem Mater 15:1617

    Article  CAS  Google Scholar 

  16. Zhang YJ, Shen YF, Han DX, Wang ZJ, Song JX, Li F, Niu L (2007) CNTs and GOx bionanocomposite bridged by ionic liquid-like unit: Preparation and electrochemical properties. Biosens Bioelectron 23:438

    Article  Google Scholar 

  17. Kaushik A, Khan R, Solanki RP, Pandey P, Alam J, Ahmad S, Malhotra BD (2008) Fe3O4 nanoparticles-CS composite based glucose biosensor. Biosens Bioelectron 24:676

    Article  CAS  Google Scholar 

  18. Wang Y, Wei WZ, Zeng JX, Liu XY, Zeng XD (2008) Fabrication of a Cu/CS/CNTs-modified glassy carbon electrode for electrochemical sensing of hydrogen peroxide and glucose. Microchim Acta 160:253

    Article  CAS  Google Scholar 

  19. Qiu JD, Xie HY, Liang RP (2008) Preparation of porous CS/CNTs film modified electrode for biosensor application. Microchim Acta 162:57

    Article  CAS  Google Scholar 

  20. Zeng JX, Wei WZ, Liu XY, Wang Y, Luo GM (2008) A simple method to fabricate a Prussian Blue nanoparticles/CNTs/poly(1,2-diaminobenzene) based glucose biosensor. Microchim Acta 160:261

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by the NNSF of China (20675064), the Ministry of Education of China (Project 708073), the Natural Science Foundation of Chongqing City (CSTC-2009BA1003) and High Technology Project Foundation of Southwest University (XSGX02), China.

Author information

Authors and Affiliations

  1. Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education and Key Laboratory of Eco-environments in Three Gorges Reservoir Region, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, People’s Republic of China

    Jingjing Li, Ruo Yuan & Yaqin Chai

Authors
  1. Jingjing Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ruo Yuan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yaqin Chai
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ruo Yuan.

Electronic supplementary materials

Below is the link to the electronic supplementary material.

ESM 1

(DOC 392 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Li, J., Yuan, R. & Chai, Y. Simple construction of an enzymatic glucose biosensor based on a nanocomposite film prepared in one step from iron oxide, gold nanoparticles, and chitosan. Microchim Acta 173, 369–374 (2011). https://doi.org/10.1007/s00604-011-0544-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00604-011-0544-0

Keywords

Search

Navigation