Abstract
A biomimetic sensor for norfloxacin is presented that is based on host-guest interactions and potentiometric transduction. The artificial host was imprinted into polymers made from methacrylic acid and/or 2-vinyl pyridine. The resulting particles were entrapped in a plasticized poly(vinyl chloride) (PVC) matrix. The sensors exhibit near-Nernstian response in steady state evaluations, and detection limits range from 0.40 to 1.0 μg mL−1, respectively, and are independent of pH values at between 2 and 6, and 8 and 11, respectively. Good selectivity was observed over several potential interferents. In flowing media, the sensors exhibit fast response, a sensitivity of 68.2 mV per decade, a linear range from 79 μM to 2.5 mM, a detection limit of 20 μg mL−1, and a stable baseline. The sensors were successfully applied to field monitoring of norfloxacin in fish samples, biological samples, and pharmaceutical products.
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Acknowledgements
The authors acknowledge the financial support from FCT, Fundação para a Ciência e Tecnologia, and FEDER by means of project PTDC/AGR-AAM/68359/2006.
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Fig. S1
Binding isotherm (A) and Scatchard plot (B) for NOR/MAA imprinted polymer. Q is the amount of NOR bond to 20.0 mg of polymer; t = 25 °C; V = 10.00 mL; binding time: 20 h (DOC 57 kb)
Fig. S2
Effect of loop and flow rate at relevant flow parameters (DOC 72 kb)
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Moreira, F.T.C., Freitas, V.A.P. & Sales, M.G.F. Biomimetic norfloxacin sensors made of molecularly-imprinted materials for potentiometric transduction. Microchim Acta 172, 15–23 (2011). https://doi.org/10.1007/s00604-010-0464-4
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DOI: https://doi.org/10.1007/s00604-010-0464-4