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Suspensions of biological tissues in alkaline medium for the determination of copper, manganese and cobalt by electrothermal atomic absorption spectrometry

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Abstract

Electrothermal atomic absorption spectrometry was applied to the determination of copper, manganese and cobalt in biological tissues of which small amounts are available only. The samples (10 mg of mice tissue) were suspended in 1 mL of water containing tetramethylammonium hydroxide, Triton X-100 and silicone antifoam. The sample was homogenized by heating to 60 °C for 5 min, submitted to dilution if necessary, and injected into the atomizer. Wall atomization was used for Cu and Mn, and platform atomization for Co. Calibration was carried out using aqueous standards for copper and manganese determination. The determination of cobalt suffered from a matrix effect and, in this case, calibration was enabled by using a suspension of a cobalt-free muscle sample. The detection limits are 25, 20 and 62 ng g−1. The results obtained for five certified reference materials demonstrated the reliability of the procedures for the determination of the three analytes in normal and dystrophic mice tissue.

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References

  1. Holm RH, Kennepohl P, Solomon EI (1996) Structural and functional aspects of metal sites in biology. Chem Rev 96:2239

    Article  CAS  Google Scholar 

  2. Degtyarenko K (2000) Bioinorganic motifs: towards functional classification of metalloproteins. Bioinformatics 16:851

    Article  CAS  Google Scholar 

  3. McCord JM, Fridovich I (1969) Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). J Biol Chem 244:6049

    CAS  Google Scholar 

  4. Wispe JR, Warner BB, Clark JC, Dey CR, Neuman J, Glasser SW, Crapo JD, Chang LY, Whitsett JA (1992) Human Mn-superoxide dismutase in pulmonary epithelial cells of transgenic mice confers protection from oxygen injury. J Biol Chem 267:23937

    CAS  Google Scholar 

  5. Roth JR, Lawrence JG, Bobik TA (1996) Cobalamin (coenzyme B-12): synthesis and biological significance. Annu Rev Microbiol 50:137

    Article  CAS  Google Scholar 

  6. Kobayashi M, Shimizu S (1999) Cobalt proteins. Eur J Biochem 261:1

    Article  CAS  Google Scholar 

  7. Bush AI (2000) Metals and neuroscience. Curr Opin Chem Biol 4:184

    Article  CAS  Google Scholar 

  8. Thackray AM, Knight R, Haswell SJ, Bujdoso R, Brown DR (2002) Metal imbalance and compromised antioxidant function are early changes in prion disease. Biochem J 362:253

    Article  CAS  Google Scholar 

  9. Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazura M (2006) Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact 160:1

    Article  CAS  Google Scholar 

  10. Shi W, Chance MR (2008) Metallomics and metalloproteomics. Cell Mol Life Sci 65:3040

    Article  CAS  Google Scholar 

  11. Tan Y, Blais JS, Marshal WD (1996) Slurry preparation by high-pressure homogenization for the determination of heavy metals in zoological and botanical feeds by electrothermal atomic absorption spectrometry. Analyst 121:1419

    Article  CAS  Google Scholar 

  12. Silva RGL, Willie SN, Sturgeon RE, Santelli RE, Sella SM (1999) Alkaline solubilization of biological materials for trace element analysis by electrothermal atomic absorption spectrometry. Analyst 124:1843

    Article  CAS  Google Scholar 

  13. Martins P, Pozebon D, Dressler VL, Kemieciki GA (2002) Determination of trace elements in biological materials using tetramethylammonium hydroxide for sample preparation. Anal Chim Acta 470:195

    Article  CAS  Google Scholar 

  14. Kotulanová A, Komárek J (2002) Determination of copper in biological materials by atomic absorption spectrometry. Acta Chim Slov 49:437

    Google Scholar 

  15. Santos Júnior D, Barbosa Júnior F, Simião de Souza S, Krug FJ (2003) Cryogenic sample grinding for copper, lead and manganese determination in human teeth by slurry sampling GFAAS. J Anal At Spectrom 18:939

    Article  Google Scholar 

  16. Taylor A, Branch S, Halls D, Patriarca M, White M (2004) Atomic spectrometry update. Clinical and biological materials, food and beverages. J Anal At Spectrom 19:505

    Article  CAS  Google Scholar 

  17. Acar O (2005) Determination of lead, chromium, manganese and zinc in slurries of botanical and biological samples by electrothermal atomic absorption spectrometry using tungsten-containing chemical modifiers. Microchim Acta 151:53

    Article  CAS  Google Scholar 

  18. Ribeiro AS, Antunes Vieira M, Furtado da Silva A, Gallingo Borges DL, Welz B, Heitmann U, Curtius AJ (2005) Determination of cobalt in biological samples by line-source and high-resolution continuum source graphite furnace atomic absorption spectrometry using solid sampling or alkaline treatment. Spectrochim Acta B 60:693

    Article  Google Scholar 

  19. Shuttler IL, Delves HT (1986) Determination of lead in blood by atomic absorption spectrometry with electrothermal atomisation. Analyst 111:651

    Article  CAS  Google Scholar 

  20. Nóbrega JA, Santos MC, de Sousa RA, Cadore S, Barnes RM, Tatro M (2006) Sample preparation in alkaline media. Spectrochim Acta B 61:465

    Article  Google Scholar 

  21. Muñoz-Delgado E, Morote-García JC, Romero-Romero R, López-García I, Hernández-Córdoba M (2006) Determination of zinc in tissues of normal and dystrophic mice using electrothermal atomic absorption spectrometry and slurry sampling. Anal Biochem 348:64

    Article  Google Scholar 

  22. Karadjova IB, Petrov PK, Serafimovski I, Stafilov T, Tsalev DL (2007) Arsenic in marine tissues-The challenging problems to electrothermal and hydride generation atomic absorption spectrometry. Spectrochim Acta B 62:258

    Article  Google Scholar 

  23. Viñas P, Pardo-Martínez M, Hernández-Córdoba M (2000) Determination of copper, cobalt, nickel, and manganese in baby food slurries using electrothermal atomic absorption spectrometry. J Agric Food Chem 48:5789

    Article  Google Scholar 

  24. Campillo N, Viñas P, López-García I, Hernández-Córdoba M (2000) Determination of arsenic in biological fluids by electrothermal atomic absorption spectrometry. Analyst 125:313

    Article  CAS  Google Scholar 

  25. Campillo N, Viñas P, López-García I, Hernández-Córdoba M (2000) Selenium determination in biological fluids using Zeeman background correction electrothermal atomic absorption spectrometry. Anal Biochem 280:195

    Article  CAS  Google Scholar 

  26. Halls DJ (1995) Analytical minimalism applied to the determination of trace element by atomic spectroscopy. J Anal At Spectrom 10:169

    Article  CAS  Google Scholar 

  27. Miller JN, Miller JC (2001) Statistics and Chemometrics for Analytical Chemistry. Prentice-Hall, Englewood Cliffs

    Google Scholar 

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Acknowledgments

Financial support from the Spanish MICINN (Project CTQ2009-08267) and from Comunidad Autónoma de la Región de Murcia (CARM. Fundación Séneca, Project 11796/PI/09) is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, E-30071, Murcia, Spain

    Natalia Campillo, Ignacio López-García, Yolanda Baeza-Albarracín & Manuel Hernández-Córdoba

  2. Department of Biochemistry and Molecular Biology A, Faculty of Biology, University of Murcia, E-30071, Murcia, Spain

    Encarnación Muñoz-Delgado

Authors
  1. Natalia Campillo
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  2. Encarnación Muñoz-Delgado
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  3. Ignacio López-García
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  4. Yolanda Baeza-Albarracín
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  5. Manuel Hernández-Córdoba
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Correspondence to Manuel Hernández-Córdoba.

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Campillo, N., Muñoz-Delgado, E., López-García, I. et al. Suspensions of biological tissues in alkaline medium for the determination of copper, manganese and cobalt by electrothermal atomic absorption spectrometry. Microchim Acta 171, 71–79 (2010). https://doi.org/10.1007/s00604-010-0411-4

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  • DOI: https://doi.org/10.1007/s00604-010-0411-4

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