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Electroanalytical performance of self-assembled monolayer gold electrode for chloramphenicol determination

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Abstract

Monolayers of 2-mercapto-5-methylbenzimidazole (MMB) were prepared on a polycrystalline gold electrode via a self-assembly process to produce a self-assembled monolayer. The resulting electrode was investigated by cyclic voltammetry and electrochemical impedance spectroscopy, and applied to the determination of chloramphenicol (CAP) in a pharmaceutical formulation using flow injection analysis along with amperometric detection. The amperometric cell was operated at −0.75 V (vs Ag/AgCl) at a flow rate of 3 mL min−1. The method was applied to the determination of CAP in ophthalmic solutions, and its performance was compared to a previously validated HPLC method. The response to CAP is linear in the range from 0.050 to 1.000 µmol L−1 (r = 0.9990), and the limit of detection is 44 µmol L−1.

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Acknowledgements

The authors gratefully acknowledge the financial support from FAPESP and thank Professor C.H. Collins for language assistance.

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Authors and Affiliations

  1. Universidade Camilo Castelo Branco—Unicastelo, Rodovia Presidente Dutra km 138, 12247004, São José dos Campos, SP, Brazil

    Lúcia Codognoto

  2. Instituto Nacional da Propriedade Industrial, Praça Mauá, 07 Centro, 20081-240, Rio de Janeiro, RJ, Brazil

    Eduardo Winter

  3. Institute of Chemistry, University of Campinas—UNICAMP, P.O. Box. 6154, 13084-971, Campinas, SP, Brazil

    Keity Margareth Doretto, Gabriel Bezzan Monteiro & Susanne Rath

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  1. Lúcia Codognoto
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  2. Eduardo Winter
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  4. Gabriel Bezzan Monteiro
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  5. Susanne Rath
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Correspondence to Susanne Rath.

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Codognoto, L., Winter, E., Doretto, K.M. et al. Electroanalytical performance of self-assembled monolayer gold electrode for chloramphenicol determination. Microchim Acta 169, 345–351 (2010). https://doi.org/10.1007/s00604-010-0339-8

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  • DOI: https://doi.org/10.1007/s00604-010-0339-8

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