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Hydrodynamic sequential injection system for a rapid dichlorophenol indophenol precipitation test for hemoglobin E

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Abstract

Dichlorophenol indophenol precipitation (DCIP) test is a screening method for unstable hemoglobins (Hb). An automatic system for a rapid DCIP test was developed based on stopped-flow hydrodynamic sequential injection. The system overcomes the problems of the conventional DCIP test such as long analysis time and imprecision, which are due to temperature fluctuation and imprecise detection timing. The flow based DCIP system can shorten the incubation time (usually 25 min to 1 h) to 3 min by using a higher temperature of 50°C instead of 37–40°C as in the conventional method. The reagents used were very simple and the whole process was done online with a microcontroller for controlling time. The clearness of the mixture was monitored with a spectrophotometer to improve precision as compared to the bare eyes observation method used in conventional DCIP test. The system could differentiate the HbE carriers (positive group) from negative group which agreed with the routine screening method using anion exchange micro-column.

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Acknowledgement

The authors thank the Commission on Higher Education (CHE) through Research Group (RG) grant for support. Additional supports from the Center for Innovation in Chemistry (PERCH-CIC) is also acknowledged.

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Authors and Affiliations

  1. Department of Chemistry and Center for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand

    Supaporn Kradtap Hartwell, Warisra Kochasit, Sutthichat Kerdphon, Jaroon Jakmunee, Somchai Lapanantnoppakhun & Kate Grudpan

  2. Department of Pediatrics, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand

    Torpong Sanguansermsri

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  1. Supaporn Kradtap Hartwell
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  2. Warisra Kochasit
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  3. Sutthichat Kerdphon
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Correspondence to Supaporn Kradtap Hartwell.

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Hartwell, S.K., Kochasit, W., Kerdphon, S. et al. Hydrodynamic sequential injection system for a rapid dichlorophenol indophenol precipitation test for hemoglobin E. Microchim Acta 167, 201–209 (2009). https://doi.org/10.1007/s00604-009-0230-7

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  • DOI: https://doi.org/10.1007/s00604-009-0230-7

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