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Preparation of a novel molecularly imprinted polymer by a sol-gel process for on-line solid-phase extraction coupled with high performance liquid chromatography to detect trace enrofloxacin in fish and chicken samples

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Abstract

A molecularly imprinted functionalized sol-gel has been prepared by using enrofloxacin (ENRO) as a template, 3-aminopropyltriethoxysilane as the functional monomer, tetraethoxysilicane as a cross-linker. This ENRO-imprinted polymer was evaluated by static, kinetic adsorption and selective experiments. The polymer displays good selectivity, and fast kinetics in terms of adsorption and desorption. The material was applied as a sorbent for on-line determination of trace ENRO by high performance liquid chromatography, with a focus on biological matrices. At a loading flow rate of 1.0 mL min-1 for sampling 50 mL, the enrichment factor and limit of detection (LOD; at S/N = 3) are 566 and 8 ng L-1, respectively. The peak area precision (the LOD for nine replicate detections of 0.1 µg L-1 ENRO) is 4.4%. ENRO in fish and chicken muscle was determined at three spiked levels with recoveries ranging from 70% to 82%.

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Acknowledgement

The authors are grateful for the financial support from the Ministry of Science and Technology of China (Project No. 2006AA10Z448 and 2008AA10Z420) and the National Natural Science Foundation of China (Project No. 20775054).

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Authors and Affiliations

  1. Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin, 300457, China

    Wang Junping, Pan Mingfei, Fang Guozhen & Wang Shuo

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  1. Wang Junping
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  2. Pan Mingfei
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  3. Fang Guozhen
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  4. Wang Shuo
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Correspondence to Wang Shuo.

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FigS1

The results of absorption tests (DOC 30 kb)

FigS2

Optimazation of preconcentration factors of on-line SPE-HPLC determination of ENRO (DOC 30 kb)

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Junping, W., Mingfei, P., Guozhen, F. et al. Preparation of a novel molecularly imprinted polymer by a sol-gel process for on-line solid-phase extraction coupled with high performance liquid chromatography to detect trace enrofloxacin in fish and chicken samples. Microchim Acta 166, 295–302 (2009). https://doi.org/10.1007/s00604-009-0205-8

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  • DOI: https://doi.org/10.1007/s00604-009-0205-8

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