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Electrochemical studies of calcium dobesilate and interaction with DNA

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Abstract

The electrochemical behavior of calcium dobesilate (CD) and its interaction with DNA were explored using voltammetry and UV spectroscopy. The results show that CD could interact with DNA molecules by intercalation, forming a non-electroactive complex. CD has excellent electrochemical activity on a gold nanoparticle-modified glassy carbon electrode with a couple of redox peaks. In the presence of DNA, the peak current of CD decreases and the peak potential is shifted positively, but no new peak appears. The binding of CD with DNA, analyzed in terms of the cooperative Hill model, yields an association constant of 2.63 × 103 L·mol−1 and a Hill coefficient of m ≈ 2. These results may serve as a reference for in vivo investigation of the interaction of CD with DNA base pairs in living cells.

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Acknowledgements

The financial support from the Ministry of Science and Technology of China (Grant No. 2007CB607606, in the name of “973” Plan) is acknowledged. The authors would like to thank Dr. Laigui Yu for his kind help.

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Authors and Affiliations

  1. Key Laboratory of Ministry of Education for Special Functional Materials, Kaifeng, 475004, People’s Republic of China

    Hui Xu, Xiaohong Li, Jingwei Zhang, Zhijun Zhang & Kuaizhi Liu

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  1. Hui Xu
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  2. Xiaohong Li
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  3. Jingwei Zhang
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  4. Zhijun Zhang
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  5. Kuaizhi Liu
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Correspondence to Kuaizhi Liu.

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Xu, H., Li, X., Zhang, J. et al. Electrochemical studies of calcium dobesilate and interaction with DNA. Microchim Acta 165, 415–420 (2009). https://doi.org/10.1007/s00604-009-0155-1

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  • DOI: https://doi.org/10.1007/s00604-009-0155-1

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