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Fabrication of CdSe and methylene blue multilayer film for the determination of adenine and guanine in DNA

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Abstract

The fabrication of an electrode modified with cadmium selenide/methylene blue{CdSe/MB}n multilayer films is introduced via assembling layer-by-layer CdSe and methylene blue on the glassy carbon electrode surface. The multilayer films have been investigated by scanning electron microscopy, electrochemical impedance spectroscopy and cyclic voltammetry. The electrode exhibited a good stability for the simultaneous determination of guanine and adenine in DNA. The experimental parameters were optimized and the limit of detection (S/N = 3) for guanine and adenine was 0.3 ng·mL−1 and 0.9 ng·mL−1, respectively, under the optimum conditions. A direct electrochemical method for simultaneous determination of guanine and adenine in DNA is presented.

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References

  1. Lvov Y (2001) In: Nalwa RW (ed) Handbook of surfaces and interfaces of materials, vol 3. Academic Press, San Diego, CA, p 170

    Google Scholar 

  2. Lvov Y (2000) In: Lvov Y, Mohwald H (eds) Protein architecture: interfacing molecular assemblies and immobilization biotechnology. Marcel Dekker, New York, p 125

    Google Scholar 

  3. Rusling JF (2000) In: Lvov Y, Mohwald H (eds) Protein architecture: interfacing molecular assemblies and immobilization biotechnology. Marcel Dekker, New York, p 337

    Google Scholar 

  4. Rusling JF, Zhang Z (2001) In: Nalwa RW (ed) Handbook of surfaces and interfaces of materials, biomolecules, biointerfaces, and applications vol 5. Academic Press, San Diego, CA, p 33

    Google Scholar 

  5. Wang HS, Ju HX, Chen HY (2002) Simultaneous determination of guanine and adenine in DNA using an electrochemically pretreated glassy carbon electrode. Anal Chim Acta 461:243

    Article  CAS  Google Scholar 

  6. Kuroda N, Nakashima K, Akiyama S (1993) Chemiluminescence method for the determination of adenine after reaction with phenylglyoxal. Anal Chim Acta 278:275

    Article  CAS  Google Scholar 

  7. Tseng HC, Dadoo C, Zare RN (1994) Selective determination of adenine containing compounds by capillary electrophoresis with laser-induced fluorescence detection. Anal Biochem 222:55

    Article  CAS  Google Scholar 

  8. Xu DK, Hua L, Chen HY (1996) Determination of purine bases by capillary zone electrophoresis with wall-jet amperometric detection. Anal Chim Acta 335:95

    Article  CAS  Google Scholar 

  9. Jin W, Wei H, Zhao X (1997) Determination of adenine and guanine by capillary zone electrophoresis with end-column amperometric detection at a carbon fiber microdisk array electrode. Electroanalysis 9:770

    Article  CAS  Google Scholar 

  10. Wang ZH, Xiao SF, Chen Y (2006) β-Cyclodextrin incorporated carbon nanotubes-modified electrodes for simultaneous determination of adenine and guanine. J Electroanal Chem 589:237

    Article  CAS  Google Scholar 

  11. Wu KB, Fei JJ, Bai W, Hu SS (2003) Direct electrochemistry of DNA, guanine and adenine at a nanostructured film-modified electrode. Anal Bioanal Chem 376:205

    CAS  Google Scholar 

  12. Wu K, Fei J, Bai W, Hu S (2003) Direct electrochemistry of DNA, guanine and adenine at a nanostructured film-modified electrode. Anal Bioanal Chem 376:205

    CAS  Google Scholar 

  13. Yao H, Li N, Xu S, Xu JZ, Zhu JJ, Chen HY (2005) Electrochemical study of a new methylene blue/silicon oxide nanocomposition mediator and its application for stable biosensor of hydrogen peroxide. Biosens Bioelectron 21:372

    Article  CAS  Google Scholar 

  14. Xiao MW, Wang LS, Wu YD, Huang XJ, Dang Z (2008) Electrochemical study of methylene blue/titanate nanotubes nanocomposite and its layer-by-layer assembly multilayer films. J Solid State Electrochem 12:1159

    Article  CAS  Google Scholar 

  15. Chai R, Yuan R, Chai Y (2008) Amperometric immunosensors based on layer-by-layer assembly of gold nanoparticles and methylene blue on thiourea modified glassy carbon electrode for determination of human chorionic gonadotrophin. Talanta 74:1330

    Article  CAS  Google Scholar 

  16. Wei X, Cruz J, Gorski W (2002) Progress in analytical chemistry of Chitosan and its derivatives. Anal Chem 74:5039

    Article  CAS  Google Scholar 

  17. Dmitry A, Claudia Q, Yann K, Bruno J, Renaud D, Emanuela R, Peter R, Adam P (2008) Oligothiophene-functionalized CdSe nanocrystals: preparation and electrochemical properties. Microchim. Acta 160:335–344

    Article  Google Scholar 

  18. Chen Z, Zhu J, Zhu JJ (2002) Preparation of submicro spheres CdSe by hydrothermal thermal process and observed by TEM. J Chin Electron Microsc Soc 21(5):619

    Google Scholar 

  19. Liu H, Wang G, Chen D, Zhang W, Li C, Fang B (2008) Fabrication of polythionine/NPAu/MWNTs modified electrode for simultaneous determination of adenine and guanine in DNA. Sens Actuator B: Chem 128:414

    Article  Google Scholar 

  20. Lesser C, Gao M, Kirstein S (1999) Highly luminescent thin films from alternating deposition of CdTe nanoparticles and polycations. Mat Sci Eng C 8–9:159

    Article  Google Scholar 

  21. Davidson N (1972) The Biochemistry of the Nucleic Acids.7th ed. Cox & Nyman, Norfolk, UK, p 129

    Google Scholar 

Download references

Acknowledgements

This work was supported by the National Natural Science Foundation and Program for Innovative Research Team in Anhui Normal University.

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Authors and Affiliations

  1. Anhui key laboratory of Chem-Biosensing, School of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241000, People’s Republic of China

    Hong Zhu & Guang-Chao Zhao

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  1. Hong Zhu
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  2. Guang-Chao Zhao
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Correspondence to Guang-Chao Zhao.

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Zhu, H., Zhao, GC. Fabrication of CdSe and methylene blue multilayer film for the determination of adenine and guanine in DNA. Microchim Acta 165, 329–334 (2009). https://doi.org/10.1007/s00604-009-0138-2

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  • DOI: https://doi.org/10.1007/s00604-009-0138-2

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