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Determination of selenomethionine in selenium-enriched yeast using capillary electrophoresis on-line coupled with electrochemiluminescence detection

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Abstract

A new method for the determination of selenomethionine (SeMet) was developed using capillary electrophoresis on-line coupled with electrochemiluminescence detection. The effects of several factors such as detection potential, concentration and pH of phosphate buffer, separation voltage and injection voltage were investigated. Under optimal conditions, the linear concentration range for SeMet was 0.001 to 0.8 μg mL−1 with a correlation coefficient of 0.9996. The limit of detection (S/N = 3) was 0.39 ng mL−1. The recovery of SeMet in selenium-enriched yeast (SEY) was 98.6% on an average. The method presented is simple, quick, sensitive and can be reliably applied to the determination of SeMet in SEY.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (Grant No. 20565001) and the Innovation Project of Guangxi Graduate Education (Grant No. 2007106020703M61).

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Authors and Affiliations

  1. Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, 541004, China

    Biyang Deng, Aihong Shi, Linqiu Li & Fang Xie

  2. Department of Material and Chemical Engineering, Guilin University of Technology, Guilin, 541004, China

    Biyang Deng, Hua Lu & Quanxiu Xu

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  1. Biyang Deng
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  2. Aihong Shi
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  3. Linqiu Li
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  4. Fang Xie
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  5. Hua Lu
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Correspondence to Biyang Deng.

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Deng, B., Shi, A., Li, L. et al. Determination of selenomethionine in selenium-enriched yeast using capillary electrophoresis on-line coupled with electrochemiluminescence detection. Microchim Acta 165, 279–283 (2009). https://doi.org/10.1007/s00604-008-0129-8

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  • DOI: https://doi.org/10.1007/s00604-008-0129-8

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