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A non-enzyme hydrogen peroxide sensor based on core/shell silica nanoparticles using synchronous fluorescence spectroscopy

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Abstract

In our previous study, we have prepared aminated fluorescent silica nanoparticles doped with fluorescein isothiocyanate (FITC) (FSNPs) for the sensing of γ-globulin. Compared with conventional organic dyes, FSNPs show superiorities such as excellent photostability, good water solubility, and biocompatibility, which are in favor of improving the stability and sensitivity of sensors. To extend the application of FSNPs, a convenient and effective method for non-enzyme fluorescent sensor of hydrogen peroxide (H2O2) is introduced based on the synchronous fluorescence technique. The sensor includes two-step reactions, typical redox reaction between KI and H2O2 and iodination reaction between I2 produced by the first step reaction and FITC doped in the network of silica nanoparticles, which induce the fluorescence quenching of FSNPs. The results show that the fluorescence signal of FSNPs linearly decreases with the trace amounts of hydrogen peroxide added in the range 5–80 μM with a detection limit of 0.8 μM under the optimal experimental conditions. The method is simple and sensitive and can be applied to the determination of trace amounts of H2O2. Good recovery data were obtained for the assay of H2O2 in river water by standard addition method with high accuracy and reliability.

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Acknowledgements

The authors are grateful for the financial support from the Natural Science Foundation of China (20705001, 20575001), the Key Project of Educational Committee of Anhui Province (KJ2007A008), the Innovation Group Project of Anhui Normal University and Startup Foundation for PhD of Anhui Normal University.

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Authors and Affiliations

  1. Anhui Key Laboratory of Chemo/biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241000, People’s Republic of China

    Fabao Luo, Jun Yin, Feng Gao & Lun Wang

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  1. Fabao Luo
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  2. Jun Yin
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  3. Feng Gao
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  4. Lun Wang
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Correspondence to Feng Gao.

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Luo, F., Yin, J., Gao, F. et al. A non-enzyme hydrogen peroxide sensor based on core/shell silica nanoparticles using synchronous fluorescence spectroscopy. Microchim Acta 165, 23–28 (2009). https://doi.org/10.1007/s00604-008-0091-5

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  • DOI: https://doi.org/10.1007/s00604-008-0091-5

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