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A reagentless nitrite biosensor based on direct electron transfer of hemoglobin on a room temperature ionic liquid/carbon nanotube-modified electrode

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Abstract

A novel amperometric biosensor for nitrite was developed by immobilization of hemoglobin (Hb) and a room temperature ionic liquid, 1-ethyl-3-methyl imidazolium tetrafluoroborate (EMIT), on a multi-walled carbon nanotubes (MWNTs) modified electrode. Compared with Hb/MWNTs modified electrode, the Hb/RTILs/MWNTs modified electrode showed better electrochemical response, indicating that EMIT can promote direct electron transfer of Hb. A pair of well-defined, quasi-reversible redox peaks of Hb with a formal potential of −0.315 V was observed. The immobilized Hb exhibited remarkable electrocatalytic activity for the reduction of nitrite. The linear response range was from 4.0 × 10−6 to 3.2 × 10−4 M with a detection limit of 8.1 × 10−7 M at a signal-to-noise ratio of 3. The resulting biosensor has been successfully applied to the determination of nitrite in water samples.

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Acknowledgements

The authors thank Professor T. Ohsaka of Tokyo Institute of Technology for providing RTIL, EMIT. This work was supported by the National Natural Science Foundation and Program for Innovative Research Team in Anhui Normal University.

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Authors and Affiliations

  1. School of Environmental Science, Anhui Normal University, Wuhu, 241003, People’s Republic of China

    Wei Wei, Hui-Hui Jin & Guang-Chao Zhao

  2. Anhui Key Laboratory of Chem-Biosensing, School of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241000, People’s Republic of China

    Guang-Chao Zhao

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  1. Wei Wei
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  2. Hui-Hui Jin
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Correspondence to Guang-Chao Zhao.

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Wei, W., Jin, HH. & Zhao, GC. A reagentless nitrite biosensor based on direct electron transfer of hemoglobin on a room temperature ionic liquid/carbon nanotube-modified electrode. Microchim Acta 164, 167–171 (2009). https://doi.org/10.1007/s00604-008-0053-y

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  • DOI: https://doi.org/10.1007/s00604-008-0053-y

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