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Amine oxidase amperometric biosensor coupled to liquid chromatography for biogenic amines determination

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Abstract

A selective and sensitive method is presented for biogenic amines (BA) determination. The novelty consists in coupling a highly selective electrochemical biosensor to a weak acid cation-exchange column for online detection of amines. A bienzyme design, based on a recently isolated amine oxidase from grass pea and commercial horseradish peroxidase, was used for the biosensor construction. The enzymes were co-immobilized on the surface of a graphite electrode together with the electrochemical mediator (Os-redox polymer). The electrochemical detection was performed at a low applied potential (−50 mV vs. Ag/AgCl, KCl0.1 M), where biases from interferences are minimal. The separation and determination of six BA, with relevance in food analysis (tyramine, putrescine, cadaverine, histamine, agmatine and spermidine), were investigated. Irrespective of the BA nature, the amine oxidase-based biosensor showed a linear response up to 5 mM, and its sensitivity decreases in the following order: cadaverine, putrescine, spermidine, agmatine, histamine and tyramine. The approach was used to estimate the BA content in fish samples, after their extraction with methanesulfonic acid.

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References

  1. Silla Santos MH (1996) Biogenic amines: their importance in foods. Int J Food Microbiol 29:213

    Article  CAS  Google Scholar 

  2. Shalaby AR (1996) Significance of biogenic amines to food safety and human health. Food Res Int 29:675

    Article  CAS  Google Scholar 

  3. Veciana-Nogues MT, Marine-Font A, Vidal-Carou MC (1997) Biogenic amines as hygienic quality indicators of tuna. Relationships with microbial counts, ATP-related compounds, volatile amines, and organoleptic changes. J Agri Food Chem 45:2036

    Article  CAS  Google Scholar 

  4. Lehane L, Olley J (2000) Histamine fish poisoning revisited. Int J Food Microbiol 58:1

    Article  CAS  Google Scholar 

  5. Tsai Y-H, Lin C-Y, Chang S-C, Chen H-C, Kung H-F, Wei C-I, Hwang D-F (2005) Occurrence of histamine and histamine-forming bacteria in salted mackerel in Taiwan. Food Microbiol 22:461

    Article  CAS  Google Scholar 

  6. Pastore P, Favaro G, Badocco D, Tapparo A, Cavalli S, Saccani G (2005) Determination of biogenic amines in chocolate by ion chromatographic separation and pulsed integrated amperometric detection with implemented wave-form at Au disposable electrode. J Chromatogr A 1098:111

    Article  CAS  Google Scholar 

  7. Halasz A, Barath A, Simon-Sarkadi L, Holzapfel W (1994) Biogenic amines and their production by microorganisms in food. Trends Food Sci Techol 5:42

    Article  CAS  Google Scholar 

  8. McCabe-Sellers BJ, Staggs CG, Bogle ML (2006) Tyramine in foods and monoamine oxidase inhibitor drugs: a crossroad where medicine, nutrition, pharmacy, and food industry converge. J Food Comp Anal 19:S58

    Article  CAS  Google Scholar 

  9. Bardocz S (1995) Polyamines in food and their consequences for food quality and human health. Trends Food Sci Technol 6:341

    Article  CAS  Google Scholar 

  10. Bardocz S, Grant G, Brown DS, Ralph A, Pusztai A (1993) Polyamines in food-implications for growth and health. J Nutr Biochem 4:66

    Article  CAS  Google Scholar 

  11. Onal A (2007) A review: current analytical methods for the determination of biogenic amines in foods. Food Chem 103:1475

    Article  Google Scholar 

  12. Venugopal V (2002) Biosensors in fish production and quality control. Biosens Bioelectron 17:147

    Article  CAS  Google Scholar 

  13. Draisci R, Volpe G, Lucentini L, Cecilia A, Federico R, Palleschi G (1998) Determination of biogenic amines with an electrochemical biosensor and its application to salted anchovies. Food Chem 62:225

    Article  CAS  Google Scholar 

  14. Ruiz-Jimenez J, Luque de Castro MD (2006) Pervaporation as interface between solid samples and capillary electrophoresis. J Chromatogr A 1110:245

    Article  CAS  Google Scholar 

  15. Alberto MR, Arena ME, Manca de Nadra MC (2002) A comparative survey of two analytical methods for identification and quantification of biogenic amines. Food Control 13:125

    Article  CAS  Google Scholar 

  16. Hwang D-F, Chang S-H, Shiua C-Y, Chai T-J (1997) High-performance liquid chromatographic determination of biogenic amines in fish implicated in food poisoning. J Chromatogr B 693:23

    Article  CAS  Google Scholar 

  17. mo Dugo G, Vilasi F, La Torre GL, Pellicano TM (2006) Reverse phase HPLC/DAD determination of biogenic amines as dansyl derivatives in experimental red wines. Food Chem 95:672

    Article  CAS  Google Scholar 

  18. Shakila RJ, Vasundhara TS, Kumudavally KV (2001) A comparison of the TLC-densitometry and HPLC method for the determination of biogenic amines in fish and fishery products. Food Chem 75:255

    Article  Google Scholar 

  19. Cinquina AL, Cali A, Longo F, De Santis L, Severoni A, Abballe F (2004) Determination of biogenic amines in fish tissues by ion-exchange chromatography with conductivity detection. J Chromatogr A 1032:73

    Article  CAS  Google Scholar 

  20. Rey M, Pohl C (2003) Novel cation-exchange column for the separation of hydrophobic and/or polyvalent amines. J Chromatogr A 997:199

    Article  CAS  Google Scholar 

  21. Saccani G, Tanzi E, Pastore P, Cavalli S, Rey M (2005) Determination of biogenic amines in fresh and processed meat by suppressed ion chromatography-mass spectrometry using a cation-exchange column. J Chromatogr A 1082:43

    Article  CAS  Google Scholar 

  22. Casella IG, Gatta M, Desimoni E (2001) Determination of histamine by high-pH anion-exchange chromatography with electrochemical detection. Food Chem 73:367

    Article  CAS  Google Scholar 

  23. Innocente N, Biasutti M, Padovese M, Moret S (2007) Determination of biogenic amines in cheese using HPLC technique and direct derivatization of acid extract. Food Chem 101:1285

    Article  CAS  Google Scholar 

  24. Li J-S, Wang H, Huang K-J, Zhang H-S (2006) Determination of biogenic amines in apples and wine with 8-phenyl-(4-oxy-acetic acid N-hydroxysuccinimide ester)-4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene by high performance liquid chromatography. Anal Chim Acta 575:255

    Article  CAS  Google Scholar 

  25. Marks (Rupp) HS, Anderson CR (2005) Determination of putrescine and cadaverine in seafood (fin fish and shellfish) by liquid chromatography using pyrene excimer fluorescence. J Chromatogr A 1094:60

    Article  Google Scholar 

  26. De Borba BM, Rohrer JS (2007) Determination of biogenic amines in alcoholic beverages by ion chromatography with suppressed conductivity detection and integrated pulsed amperometric detection. J Chromatogr A 1155:1122

    Google Scholar 

  27. Favaro G, Pastore P, Saccani G, Cavalli S (2007) Determination of biogenic amines in fresh and processed meat by ion chromatography and integrated pulsed amperometric detection on Au electrode. Food Chem 105:1652

    Article  CAS  Google Scholar 

  28. Šebela M, Luhová L, Frébort I, Faulhammer HG, Hirota S, Zajoncová L, Stužka V, Peč P (1998) Analysis of the active sites of copper/topa quinone-containing amine oxidases from Lathyrus odoratus and L. sativus seedlings. Phytochem Anal 9:211

    Article  Google Scholar 

  29. Ohara TJ, Rajagopalan R, Heller A (1994) “Wired” enzyme electrodes for amperometric determination of glucose or lactate in the presence of interfering substances. Anal Chem 66:2451

    Article  CAS  Google Scholar 

  30. Niculescu M, Nistor C, Frébort I, Peč P, Mattiasson B, Csöregi E (2000) Redox hydrogel-based amperometric bienzyme electrodes for fish freshness monitoring. Anal Chem 72:1591

    Article  CAS  Google Scholar 

  31. Keunchkarian S, Reta M, Romero L, Castells C (2006) Effect of sample solvent on the chromatographic peak shape of analytes eluted under reversed-phase liquid chromatogaphic conditions. J Chromatogr A 1119:20

    Article  CAS  Google Scholar 

  32. Palmer T (1985) Understanding enzymes, 2nd edn. Ellis Horwood, Chichester, p 75

    Google Scholar 

  33. Skoog DA, Leary JJ (1992) Principles of instrumental analysis, 4th edn. Saunders College, USA, p 592

    Google Scholar 

  34. Tsai Y-H, Hsieh H-S, Chen H-C, Cheng S-H, Chai T-J, Hwang D-F (2007) Histamine level and species identification of billfish meats implicated in two food-borne poisonings. Food Chem 104:1366

    Article  CAS  Google Scholar 

Download references

Acknowledgements

Romanian National Council of Research in Universities (research grant TD 5/267-2007, L.M.) and Ministry of Education, Youth and Sport of the Czech Republic (research grant MSM619859216, I.F.) are gratefully acknowledged for financial support.

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Authors and Affiliations

  1. Department of Physical Chemistry, “Babeş-Bolyai” University, Arany Janos 11, RO-400028, Cluj-Napoca, Romania

    Laura Mureşan & Ionel Catalin Popescu

  2. Department of Analytical Chemistry, Lund University, P.O. Box 124, SE-22100, Lund, Sweden

    Laura Mureşan, Ruben Ronda Valera, Elisabeth Csöregi & Mihaela Nistor

  3. Department of Biochemistry, Palacký University, Šlechtitelů 11, CZ-78371, Olomouc, Czech Republic

    Ivo Frébort

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  1. Laura Mureşan
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  2. Ruben Ronda Valera
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  3. Ivo Frébort
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  4. Ionel Catalin Popescu
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  5. Elisabeth Csöregi
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  6. Mihaela Nistor
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Correspondence to Laura Mureşan.

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Mureşan, L., Ronda Valera, R., Frébort, I. et al. Amine oxidase amperometric biosensor coupled to liquid chromatography for biogenic amines determination. Microchim Acta 163, 219–225 (2008). https://doi.org/10.1007/s00604-008-0033-2

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  • DOI: https://doi.org/10.1007/s00604-008-0033-2

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