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Flow-injection chemiluminescence determinations for human blood lead using controlled reagent release technology

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Abstract

A flow-through CL method for the determination of lead combined with controlled-reagent-release technology has been developed. Chemiluminescence (CL) reagents luminol and potassium permanganate were immobilized on anion exchange resin by electrostatic interaction. Lead ion was determined by its enhancing effect on the CL reaction between luminol and potassium permanganate. Both luminol and potassium permanganate were eluted from the anion exchange resin column by sodium phosphate solution. The linear range of the system was 10 μg mL−1, and the detection limit was 5 × 10–9 g mL−1 lead (3σ). A complete analysis could be performed in 1 min with a relative SD 3.2% (1.0 × 10–7 g mL−1, n = 9). The column shows remarkable stability and can be reused over 350 times and 21 days. The method has been applied to determine lead in human blood samples.

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Acknowledgment

This work was financially supported by the National Science Basic Research Project of China (grant No. 30600133 and grant No. 20775047). We also acknowledged the support of Huo Yingdong Teaching Foundation of China (grant No. 81013).

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Authors and Affiliations

  1. State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Si Pai Lou 2#, Nanjing, 210096, People’s Republic of China

    Peng Qu, Shan Cheng Yan, Hua Lu & Zu Hong Lu

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  1. Peng Qu
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  2. Shan Cheng Yan
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  3. Hua Lu
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  4. Zu Hong Lu
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Correspondence to Zu Hong Lu.

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Qu, P., Yan, S.C., Lu, H. et al. Flow-injection chemiluminescence determinations for human blood lead using controlled reagent release technology. Microchim Acta 163, 321–326 (2008). https://doi.org/10.1007/s00604-008-0021-6

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  • DOI: https://doi.org/10.1007/s00604-008-0021-6

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