The effect of membrane permeability on the response of a catechol biosensor

Abstract

A mathematical model is developed for the simulation of the amperometric response of a biosensor for catechol using polyphenoloxidase. The model is based on transient diffusion equations containing nonlinear terms of Michaelis-Menten for two space regions: the diffusion layer and the biomembrane containing the immobilized enzyme. The set of partial derivatives of nonlinear equations and the corresponding boundary and initial conditions was solved using the implicit finite difference technique. This numerical solution was then exploited to study the effects of permeability and thickness of the biomembrane on the maximum response of the reduction current and the amplification factor corresponding to the maximum of catalytic activity of the enzyme. This amplification factor increases with the thickness of the biomembrane while permeability is weak. In the case of the low initial concentrations (10⁻⁶ to 5.10⁻⁴ mM), its value is maximal and remains independent of substrate concentration. Also, the amplification factor is more significant when the diffusion resistance is more important, i.e. for high thicknesses or weak permeabilities of the biomembranes.