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Sensitive chemiluminescence method for the determination of glutathione, l-cysteine and 6-mercaptopurine

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Abstract

Glutathione (GSH), l-cysteine (l-Cys) and 6-mercaptopurine (6-MP) inhibit the CL reaction of luminol–H2O2 catalyzed by gold colloids. In order to explore this, GSH, l-Cys and 6-MP were injected into the chemiluminescence system of luminol and H2O2 catalyzed by gold colloids. The results showed that gold colloids interact with GSH, l-Cys and 6-MP and decrease the CL emission. Based on this phenomenon, a simple, sensitive and convenient flow injection CL method was developed for the determination of GSH, l-Cys and 6-MP. This method provides a novel, and effective CL assay for GSH, l-Cys and 6-MP that has been applied to the determination of GSH in human serum.

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References

  1. Ames BN (1983) Dietary carcinogens and anti-carcinogens (oxygen radicals and degenerative diseases). Science 221:1256

    Article  CAS  Google Scholar 

  2. Cerutti PA (1985) Preoxidant states and tumor promotion. Science 227:375

    Article  CAS  Google Scholar 

  3. Cross CE, Halliwell B, Borish ET, Pryor WA, Ames BN, Saul RL, McCord JM, Harman D (1987) Oxygen radicals and human disease. Ann Intern Med 107:526

    CAS  Google Scholar 

  4. Blasco F, Medina-Hernandez MJ, Sagrado S (1997) Use of pH gradients in continuous-flow systems and multivariate regression techniques applied to the determination of methionine and cysteine in pharmaceuticals. Anal Chim Acta 348:151

    Article  CAS  Google Scholar 

  5. Lunar ML, Rubio S, Pereze-Bendito D, Carreto ML, Mcleod CW (1997) Hexadecylpyridinium chloride micelles for the simultaneous kinetic determination of cysteine and cystine by their induction of the iodine–azide reaction. Anal Chim Acta 337:341

    Article  Google Scholar 

  6. Toyooka T, Imai K (1984) New fluorogenic reagent having halogenobenzofurazan structure for thiols: 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole. Anal Chem 56:2461

    Article  CAS  Google Scholar 

  7. Compagnone D, Massoud R, Di Ilio C, Federici G (1991) Potentiometric determination of glutathione and glutathione transferase activity. Anal Lett 24:993

    CAS  Google Scholar 

  8. Jin W, Wang Y (1997) Determination of cysteine by capillary zone electrophoresis with end column amperometric detection at a gold/mercury amalgam microelectrode without deoxygenation. J Chromatogr A 769:307

    Article  CAS  Google Scholar 

  9. Spataru N, Sarada B V, Popa E, Tryk DA, Fujishima A (2001) Voltammetric determination of L-Cysteine at conductive diamond electrodes. Anal Chem 73:514

    Article  CAS  Google Scholar 

  10. Birwe H, Hesse A (1991) High-performance liquid chromatographic determination of urinary cysteine and cystine. Clin Chim Acta 199:33

    Article  CAS  Google Scholar 

  11. Tcherkas YV, Denisenko AD (2001) Simultaneous determination of several amino acids, including homocysteine, cysteine and glutamic acid, in human plasma by isocratic reversed-phase high-performance liquid chromatography with fluorimetric detection. J Chromatogr A 913:309

    Article  CAS  Google Scholar 

  12. Tang JL, Zhou SW, Huang LQ, Huang YP, Zhang HJ (1998) Determination of azathiopurine and 6-mercaptopurine in serum by reversed-phase high performance liquid chromatography. Chin J Chromatogr 16:513

    CAS  Google Scholar 

  13. Warren CWC, Nie S (1998) Quantum dot bioconjugates for ultrasensitvie nonisotopic detection. Science 281:2016

    Article  Google Scholar 

  14. Alivisatos AP, Johnsson KP, Peng XG, Wilson TE, Lowethm CJ, Bruchez MP, Schultz PC (1996) Organization of ‘nanocrystal molecules’ using DNA. Nature 382:609

    Article  CAS  Google Scholar 

  15. Tian Y, Tewton T, Kotov NA, Guldi DM, Fendler JH (1996) Coupled composite CdS–CdSe and core-shell types of (CdS)CdSe and (CdSe)CdS nanoparticles. J Phys Chem 100:8927

    Article  CAS  Google Scholar 

  16. Pathak S, Choi SK, Arnheim N, Thompson ME (2001) Hydroxylated quantum dots as luminescent probes for in situ hybridization. J Am Chem Soc 123:4103

    Article  CAS  Google Scholar 

  17. Agbaria RA, Oldham PB, McCarroll M, McGown LB, Warner IM (2002) Molecular fluorescence, phosphorescence, and chemiluminescence spectrometry. Anal Chem 74:3952

    Article  CAS  Google Scholar 

  18. Zhang ZF, Cui H, Lai CZ, Liu LJ (2005) Gold nanoparticle-catalyzed luminol chemiluminescence and its analytical applications. Anal Chem 77:3324

    Article  CAS  Google Scholar 

  19. Cui H, Zhang ZF, Shi MJ, Xu Y, Wu YL (2005) Light emission of gold nanoparticles induced the reaction of bis(2,4,6-trichlorophenyl) oxalate and hydrogen peroxide. Anal Chem 77:6402

    Article  CAS  Google Scholar 

  20. Cui H, Zhang ZF, Shi MJ (2005) Chemiluminescent reactions induced by gold nanoparticles. J Phys Chem B 109:3099

    Article  CAS  Google Scholar 

  21. Frens G (1973) Controlled nucleation for the regulation of the particle size in monodisperse gold suspensions. Nat Phys Sci 241:20

    CAS  Google Scholar 

  22. Zhang P, Sham TK (2003) X-ray studies of the structure and electronic behavior of alkanethiolate-capped gold nanoparticles: the interplay of size and surface effects. Phys Rev Lett 90:245502-1

    Article  Google Scholar 

  23. Ghosh SK, Nath S, Kundu S, Esumi K, Pal T (2004) Solvent and ligand effects on the localized surface plasmon resonance (LSPR) of gold colloids. J Phys Chem B 108:13963

    Article  CAS  Google Scholar 

  24. Merényi G, Lind J, Eriksen TE (1990) Luminol chemiluminescence: chemistry, excitation, emitter. J Biolumin Chemilumin 5:53

    Article  Google Scholar 

  25. Pavlov V, Xiao Y, Gill R, Dishon A, Kotler M, Willner I (2004) Amplified chemiluminescence surface detection of DNA and telomerase activity using catalytic nucleic acid labels. Anal Chem 76:2152

    Article  CAS  Google Scholar 

  26. Burdo TG, Seitz WR (1975) Mechanism of cobalt catalysis of luminol chemiluminescence. Anal Chem 47:1639

    Article  CAS  Google Scholar 

  27. Matachescu C, Kulmala S, Ala-Kleme T, Joela H (1997) Mechanism and analytical applicability of luminol-specific extrinsic lyoluminescence of UV-irradiated potassium peroxodisulfate. Anal Chem 69:3385

    Article  CAS  Google Scholar 

  28. Wang L, Yang P, Li YX, Chen HQ, Li MG, Luo FB (2007) A flow injection chemiluminescence method for the determination of fluoroquinolone derivative using the reaction of luminal and hydrogen peroxide catalyzed by gold nanoparticles. Talanta 72:1066

    Article  CAS  Google Scholar 

  29. Li YX, Yang P, Wang P, Wang L (2007) Development of a novel luminol chemiluminescent method catalyzed by gold nanoparticles for determination of estrogens. Anal Bioanal Chem 387:585

    Article  CAS  Google Scholar 

  30. Hayashi T, Tanaka K, Haruta M (1998) Selective vapor-phase epoxidation of propylene over Au/TiO2 catalysts in the presence of oxygen and hydrogen. J Catal 178:566

    Article  CAS  Google Scholar 

  31. Overbury SH, Ortiz-Soto L, Zhu HG, Lee B, Amiridis MD, Dai S (2004) Comparison of Au catalysts supported on mesoporous titania and silica: investigation of Au particle size effects and metal-support interactions. Catal Lett 95:99

    Article  CAS  Google Scholar 

  32. Ellman GL (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82:70

    Article  CAS  Google Scholar 

Download references

Acknowledgment

This work was supported financially by the Natural Science Foundation of Educational Department of Anhui Province (No. 2006kj039a), the Nature Science Foundation of Anhui Province (No.070414270X), the National Natural Science Foundation of China (No. 20575001), the Education Department of Anhui Province (No.2006KJ006TD) and a Project for the Author of Excellent Teacher of Anhui Province.

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Authors and Affiliations

  1. Department of Chemistry, Huainan Normal University, Huainan, 232001, People’s Republic of China

    Ping Yang, Yonghong Chen, Qiyong Zhu & Fengwu Wang

  2. Anhui Key Laboratory of Functional Molecular Solids, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, 24100, People’s Republic of China

    Ping Yang, Lun Wang & Yongxin Li

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  1. Ping Yang
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  2. Yonghong Chen
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  3. Qiyong Zhu
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  4. Fengwu Wang
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  5. Lun Wang
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  6. Yongxin Li
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Correspondence to Ping Yang.

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Yang, P., Chen, Y., Zhu, Q. et al. Sensitive chemiluminescence method for the determination of glutathione, l-cysteine and 6-mercaptopurine. Microchim Acta 163, 263–269 (2008). https://doi.org/10.1007/s00604-008-0006-5

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  • DOI: https://doi.org/10.1007/s00604-008-0006-5

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