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Hydrogen peroxide biosensor based on horseradish peroxidase–Au nanoparticles at a viologen grafted glassy carbon electrode

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Abstract

A novel hydrogen peroxide biosensor was fabricated that is based on horseradish peroxidase–Au nanoparticles immobilized on a viologen-modified glassy carbon electrode (GCE) by amino cation radical oxidation in basic solution. The immobilized BAPV acts as a mediator and a covalent linker between GCE and the Au nanoparticles. The biosensor exhibited fast response, good reproducibility, and long-term stability.

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Acknowledgements

This project was supported by the National Natural Science Foundation of China (No. 20645004), Changchun Institute of Applied Chemistry, and State Key Laboratory of Electroanalytical Chemistry. The author wishes to thank Professor Dong SJ for instructive advice.

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Authors and Affiliations

  1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China

    Jianbo Jia

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  1. Jianbo Jia
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Correspondence to Jianbo Jia.

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Jia, J. Hydrogen peroxide biosensor based on horseradish peroxidase–Au nanoparticles at a viologen grafted glassy carbon electrode. Microchim Acta 163, 237–241 (2008). https://doi.org/10.1007/s00604-008-0002-9

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  • DOI: https://doi.org/10.1007/s00604-008-0002-9

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