Skip to main content

Advertisement

SpringerLink
Log in

An electronic tongue for fish freshness analysis using a thick-film array of electrodes

  • Original Paper
  • Published:
Microchimica Acta Aims and scope Submit manuscript

Abstract.

We report on the development of an electronic tongue as potential tool for fish freshness determination. The studies were carried out following the evolution with time on fillet of cultured sea bream (Sparus Auratus). The electronic tongue consists of 16 potentiometric electrodes of the type metal, metal oxide and insoluble metal salts. Graphite was also used as electrode. Fish freshness indicators such as texture, pH, colour, microbial analysis, total volatile basic nitrogen (TVB-N) and biogenic amines were also determined versus time. The effectivity of the electronic tongue in the assessment of the evolution with time of fish fillets was evaluated. First the electronic tongue was used to classify samples according to time. Perceptron and adaptive resonance theory were used to discriminate between the data from different days. In a second step, the electronic tongue was used to predict the results obtained from chemical and biochemical analyses by building quantitative partial least square (PLS) models. A remarkable correlation was found between the electronic tongue formed by the 16 simple electrodes and parameters such as total biogenic amines, pH, TVB-N and microbial analysis with correlation coefficients larger than 0.98.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • I Karube H Matsuoka S Suzuki E Watanabe K Toyama (1984) ArticleTitleDetermination of fish freshness with an enzyme sensor system J Agric Food Chem 32 314 Occurrence Handle10.1021/jf00122a034 Occurrence Handle1:CAS:528:DyaL2cXhtF2ns78%3D

    Article  CAS  Google Scholar 

  • Luten B, Martinsdottir E (1997) QIM: European tool for fish freshness evaluation in the fishery chain, in Methods to Determine the Freshness of Fish in Research and Industry. International Institute of Refrigeration Paris, France

  • G Olafsdottir P Nesvadba C Di Natale M Careche J Oehlenschläger R Tryggvadóttir R Schubring M Kroeger K Heia M Esaiassen A Macagnano B Jorgensen (2004) ArticleTitleMultisensor for fish quality determination Trends Food Sci Tech 15 86 Occurrence Handle10.1016/j.tifs.2003.08.006 Occurrence Handle1:CAS:528:DC%2BD2cXptVemsg%3D%3D

    Article  CAS  Google Scholar 

  • D James S M Scott A Ali W T O’Hare (2005) ArticleTitleChemical sensors for electronic nose systems Microchimica Acta 149 1 Occurrence Handle10.1007/s00604-004-0291-6 Occurrence Handle1:CAS:528:DC%2BD2MXksFymsg%3D%3D

    Article  CAS  Google Scholar 

  • S M Scott D James Z Ali (2006) ArticleTitleData analysis for electronic nose systems Microchimica Acta 156 183 Occurrence Handle10.1007/s00604-006-0623-9 Occurrence Handle1:CAS:528:DC%2BD28XhtlaitLvO

    Article  CAS  Google Scholar 

  • R Pérez-Olmos R Herrero J L F C Lima M C B S Montenegro (1997) ArticleTitleSequential potentiometric determination of chloride and nitrate in meat products Food Chem 59 305 Occurrence Handle10.1016/S0308-8146(96)00263-4

    Article  Google Scholar 

  • N Kaneki T Miura K Shimada H Tanaka S Ito K Hotori C Akasaka S Ohkubo Y Asano (2004) ArticleTitleMeasurement of pork freshness using potentiometric sensor Talanta 62 217 Occurrence Handle10.1016/j.talanta.2003.07.002 Occurrence Handle1:CAS:528:DC%2BD3sXpvVaisL4%3D

    Article  CAS  Google Scholar 

  • P Ciosek Z Brzózka W Wróblewski (2004) ArticleTitleClassification of beverages using a reduced sensor array Sensor Actuator B 103 76 Occurrence Handle10.1016/j.snb.2004.04.038 Occurrence Handle1:CAS:528:DC%2BD2cXnsVGms7Y%3D

    Article  CAS  Google Scholar 

  • A Legin A Smirnova A M Rudnitskaya L Lvova E Suglobova Y Vlasov (1999) ArticleTitleChemical sensor array for multicomponent analysis of biological liquids Anal Chim Acta 385 131 Occurrence Handle10.1016/S0003-2670(98)00690-4 Occurrence Handle1:CAS:528:DyaK1MXhvFClu7Y%3D

    Article  CAS  Google Scholar 

  • J Gallardo S Alegret R Muñoz M De-Román L Leija P R Hernández M Del Valle (2003) ArticleTitleAn electronic tongue using potentiometric all-solid-state PVC-membrane sensors for the simultaneous quantification of ammonium and potassium ions in water Anal Bioanal Chem 377 248 Occurrence Handle10.1007/s00216-003-2042-7 Occurrence Handle1:CAS:528:DC%2BD3sXmvVKgsL0%3D

    Article  CAS  Google Scholar 

  • L Lvova E Martinelli E Mazzone A Pede R Paolesse C Di Natale A D’Amico (2006) ArticleTitleElectronic tongue based on an array of metallic potentiometric sensors Talanta 70 833 Occurrence Handle10.1016/j.talanta.2006.02.014 Occurrence Handle1:CAS:528:DC%2BD28XhtVWqt7rL

    Article  CAS  Google Scholar 

  • R H Labrador J Soto R Martínez-Máñez C Coll A Benito J Ibáñez E García-Breijo L Gil (2007) ArticleTitleAn electrochemical characterization of thick-film electrodes based on RuO2-containing resistive pastes J Electroanaly Chem 611 175 Occurrence Handle10.1016/j.jelechem.2007.08.019 Occurrence Handle1:CAS:528:DC%2BD2sXhtlChtbnJ

    Article  CAS  Google Scholar 

  • R Martínez-Máñez J Soto E García-Breijo L Gil J Ibáñez E Gadea (2005) ArticleTitleA multisensor in thick-film technology for water quality control Sensor and Actuator A 120 589 Occurrence Handle10.1016/j.sna.2005.03.006 Occurrence Handle1:CAS:528:DC%2BD2MXjslCjtLY%3D

    Article  CAS  Google Scholar 

  • S Cakli B Kilinc A Cadun T Dincer S Tolasa (2007) ArticleTitleQuality differences of whole ungutted sea bream (Saprus aurata) and sea bass (Dicentrarchus labrax) while stored in ice Food Control 18 391 Occurrence Handle10.1016/j.foodcont.2005.11.005 Occurrence Handle1:CAS:528:DC%2BD28XhtFSrur7P

    Article  CAS  Google Scholar 

  • Huss H H (1995) Quality and quality changes in fresh fish. In: Huss H H (ed) FAO Fisheries Technical Paper 348, Rome

  • J A Mihell J K Atkinson (1998) ArticleTitlePlanar thick-film pH electrodes based on ruthenium dioxide hydrate Sensor Actuator B 48 505 Occurrence Handle10.1016/S0925-4005(98)00090-2

    Article  Google Scholar 

  • J Lee R Myers A Jang P Bhadri F Beyette SuffixJr W Timmons P Bishop I Papautsky (2004) ArticleTitlePotentiometric microelectrode sensors for in situ Environ Monit Sens Proc IEEE 1 361 Occurrence Handle1:CAS:528:DC%2BD2cXmtlygsr4%3D

    CAS  Google Scholar 

  • R Martínez-Máñez J Soto E García-Breijo L Gil J Ibáñez E Llobet (2005) ArticleTitleAn “electronic tongue” design for the qualitative analysis of natural water Sensor Actuator B 104 302 Occurrence Handle10.1016/j.snb.2004.05.022 Occurrence Handle1:CAS:528:DC%2BD2cXhtVWju7vF

    Article  CAS  Google Scholar 

  • L Gil E Garcia-Breijo J Ibáñez R H Labrador E Llobet R Martínez Máñez J Soto (2006) ArticleTitleElectronic tongue for qualitative analysis of aqueous solutions of salts using thick-film technology and metal electrodes Sensors 9 1128 Occurrence Handle10.3390/s6091128

    Article  Google Scholar 

  • J I Fernández-Segovia I A Escriche A Fuentes J A Serra (2007) ArticleTitleMicrobial and sensory changes during refrigerated storage of desalted cod (Gadus morhua) preserved by combined methods Int J Food Microbiol 116 64 Occurrence Handle10.1016/j.ijfoodmicro.2006.12.026 Occurrence Handle1:CAS:528:DC%2BD2sXjslSht7c%3D

    Article  CAS  Google Scholar 

  • Y Vlasov A Legin A Rudnitskaya (1997) ArticleTitleCross-sensitivity evaluation of chemical sensors for electronic tongue: determination of heavy metal ions Sensor Actuator B 44 532 Occurrence Handle10.1016/S0925-4005(97)00241-4

    Article  Google Scholar 

  • G A Carpenter S Gossberg N Markuzon J Reynolds D Rosen (1992) ArticleTitleFuzzy Artmap: a neural network architecture for incremental supervised learning of analog multidimensional maps IEEE T Neural Networ 3 698 Occurrence Handle10.1109/72.159059 Occurrence Handle1:STN:280:DC%2BD1c7hslyksg%3D%3D

    Article  CAS  Google Scholar 

  • E Llobet E Hines J W Garder S Franco (1999) ArticleTitleNon-destructive banana ripeness determination using a neural network-based electronic nose Means Sci Technol 10 538 Occurrence Handle10.1088/0957-0233/10/6/320 Occurrence Handle1:CAS:528:DyaK1MXjslWntbw%3D

    Article  CAS  Google Scholar 

  • V P Lougovois E R Kyranas V R Kyrana (2003) ArticleTitleComparison of selected methods of assessing freshness quality and remaining storage life of iced gilthead sea bream (Sparus aurata) Food Res Int 36 551 Occurrence Handle10.1016/S0963-9969(02)00220-X

    Article  Google Scholar 

  • H Uchiyama S Ehira (1974) ArticleTitleRelation between freshness and acid-soluble nucleotides in aseptic cod and yellowtail muscles during ice storage Bulletin of Tokai Regional Fisheries Research Laboratory 78 23 Occurrence Handle1:CAS:528:DyaE2MXktVOgu7k%3D

    CAS  Google Scholar 

  • Olafsdóttir G, Lutten J, Dalgaard P, Careche M, Verrez-Bagnis V, Martinsdóttir E, Heia K, Use of counts of hydrogen sulphide producing bacteria to estimate remaining shelf life of fresh fish. Methods to determine the freshness of fish in research and industry. International Institute of Refrigeration, Paris, France, p 175

  • M Barroso M Careche A J Borderías (1998) ArticleTitleQuality control of frozen fish using rheological techniques Trends Food Sci Tech 9 223 Occurrence Handle10.1016/S0924-2244(98)00047-8 Occurrence Handle1:CAS:528:DyaK1cXns12it74%3D

    Article  CAS  Google Scholar 

  • L A Berruela R M Alonso-Salces K Héberguer (2007) ArticleTitleSupervised pattern recognition in food analysis J Chromatogr A 1158 196 Occurrence Handle10.1016/j.chroma.2007.05.024 Occurrence Handle1:CAS:528:DC%2BD2sXnsFylsL4%3D

    Article  CAS  Google Scholar 

  • M Vinaixa E Llobet J Brezmes X Vilanova X Correig (2005) ArticleTitleA fuzzy ARTMAP – and PLS-based MS e-nose for the qualitative and quantitative assessment of rancidity in crisps Sensor Actuator B 106 677 Occurrence Handle10.1016/j.snb.2004.05.038 Occurrence Handle1:CAS:528:DC%2BD2MXjtlGisb4%3D

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Instituto de Química Molecular Aplicada, Universidad Politécnica de Valencia, Valencia, Spain

    Luis Gil, Eduardo Garcia-Breijo, Ramón Martínez-Máñez & Juan Soto

  2. Department of Food Technology, Universidad Politécnica de Valencia, Valencia, Spain

    José M. Barat & Isabel Escriche

Authors
  1. Luis Gil
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. José M. Barat
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Isabel Escriche
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Eduardo Garcia-Breijo
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ramón Martínez-Máñez
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Juan Soto
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Luis Gil, José M. Barat or Juan Soto.

Additional information

Correspondence: Luis Gil, Juan Soto, Instituto de Química Molecular Aplicada, Universidad Politécnica de Valencia, Camino de Vera s/n. 46022 Valencia, Spain; José M. Barat, Department of Food Technology, Universidad Politécnica de Valencia. Camino de Vera s/n. 46022 Valencia, Spain

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gil, L., Barat, J., Escriche, I. et al. An electronic tongue for fish freshness analysis using a thick-film array of electrodes. Microchim Acta 163, 121–129 (2008). https://doi.org/10.1007/s00604-007-0934-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00604-007-0934-5

Search

Navigation