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Biochemical sniffer with choline oxidase for measurement of choline vapour

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Abstract.

An enzyme-based gas sensor (bio-sniffer) for choline vapour was fabricated and tested. The bio-sniffer was constructed using a Clark-type dissolved oxygen electrode and an enzyme (choline oxidase) immobilized membrane. This bioelectronic device measures choline concentration by the oxygen consumption induced by an enzyme reaction of choline oxidase. As the assessment of sensor performances, the calibration curves for choline in the liquid and gas phases were investigated, respectively. The responses of the bioelectronic device to choline solutions of various concentrations were related within a range from 5.00 to 700 µmol·L−1 with a correlation coefficient of 0.999. On the other hand, the bio-sniffer for choline vapour was placed into a gas-measuring chamber and calibrated using gas detection tubes. The calibration range was 1.00–30.0 ppm (correlation coefficient: 0.996). The response time for choline vapour was approximately 15% slower than that of biosensor for choline solution. These results indicate that the bio-sniffer is useful to monitor colourless and odourless choline gas released from coating compositions including choline.

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References

  • M H Faridnia G Palleschi G J Lubrano G G Guilbault (1993) ArticleTitleAmperometric biosensor for determination of lactate in sweat Anal Chim Acta 278 235 Occurrence Handle10.1016/0003-2670(93)80082-V

    Article  Google Scholar 

  • S V Dzyadevych V N Arkhypova C Martelet N Jaffrezic-Renault J M Chovelon A V El’skaya A P Soldatkin (2004) ArticleTitlePotentiometric biosensors based on ISFETs and immobilized cholinesterases Electroanalysis 16 1873 Occurrence Handle10.1002/elan.200403075 Occurrence Handle1:CAS:528:DC%2BD2cXhtVOmtL3P

    Article  CAS  Google Scholar 

  • G Cuadra K Summers E Giacobini (1994) ArticleTitleCholinesterase inhibitor effects on neurotransmitters in rat cortex in vivo J Pharmacol Exp Ther 270 277 Occurrence Handle1:CAS:528:DyaK2cXkvF2lurs%3D

    CAS  Google Scholar 

  • M G Garguilo A C Michael (1996) ArticleTitleAmperometric microsensors for monitoring choline in the extracellular fluid of brain J Neurosci Meth 70 73 Occurrence Handle10.1016/S0165-0270(96)00105-7 Occurrence Handle1:CAS:528:DyaK28XntlWisLs%3D

    Article  CAS  Google Scholar 

  • S Consolo C F Wu F Fiorentini H Ladinsky A Vezzani (1987) ArticleTitleDetermination of endogenous acetylcholine release in freely moving rats by transstriatal dialysis coupled to a radioenzymatic assay: effect of drugs J Neurochem 48 1459 Occurrence Handle10.1111/j.1471-4159.1987.tb05686.x Occurrence Handle1:CAS:528:DyaL2sXitVOrsLc%3D

    Article  CAS  Google Scholar 

  • Q Xin R M Wightman (1997) ArticleTitleTransport of choline in rat brain slices Brain Res 776 126 Occurrence Handle10.1016/S0006-8993(97)00996-7 Occurrence Handle1:CAS:528:DyaK2sXnvFGmsbo%3D

    Article  CAS  Google Scholar 

  • Q Cai K Zeng C Ruan T A Desai C A Grimes (2004) ArticleTitleA wireless, remote query glucose biosensor based on a pH-sensitive polymer Anal Chem 76 4038 Occurrence Handle10.1021/ac0498516 Occurrence Handle1:CAS:528:DC%2BD2cXktFSnurc%3D

    Article  CAS  Google Scholar 

  • N S Lawrence R P Deo J Wang (2004) ArticleTitleBiocatalytic carbon paste sensors based on a mediator pasting liquid Anal Chem 76 3735 Occurrence Handle10.1021/ac049943v Occurrence Handle1:CAS:528:DC%2BD2cXjvVCks7Y%3D

    Article  CAS  Google Scholar 

  • I Karube K Hara I Satoh S Suzuki (1979) ArticleTitleAmperometric determination of phosphatidyl choline in serum with use of immobilized phospholipase d and choline oxidase Anal Chim Acta 106 243 Occurrence Handle10.1016/S0003-2670(01)85007-8 Occurrence Handle1:CAS:528:DyaE1MXktFKisrs%3D

    Article  CAS  Google Scholar 

  • M Mascini D Moscone (1986) ArticleTitleAmperometric acetylcholine and choline sensors with immobilized enzymes Anal Chim Acta 179 439 Occurrence Handle10.1016/S0003-2670(00)84489-X Occurrence Handle1:CAS:528:DyaL28Xit1Wnurk%3D

    Article  CAS  Google Scholar 

  • J Marty K Sode I Karube (1990) ArticleTitleAmperometric determination of choline and acetylcholine with enzymes immobilized in a photocross-linkable polymer Anal Chim Acta 228 49 Occurrence Handle10.1016/S0003-2670(00)80477-8 Occurrence Handle1:CAS:528:DyaK3cXitFehsrc%3D

    Article  CAS  Google Scholar 

  • Z Huang R Villarta-snow G J Lubrano G G Guilbault (1993) ArticleTitleDevelopment of choline and acetylcholine Pt microelectrodes Anal Biochem 215 31 Occurrence Handle10.1006/abio.1993.1550 Occurrence Handle1:CAS:528:DyaK2cXmvF0%3D

    Article  CAS  Google Scholar 

  • K Kano K Morikage B Uno Y Esaka M Goto (1994) ArticleTitleEnzyme microelectrodes for choline and acetylcholine and their applications Anal Chim Acta 299 69 Occurrence Handle10.1016/0003-2670(94)00318-1 Occurrence Handle1:CAS:528:DyaK2MXitlWmt7k%3D

    Article  CAS  Google Scholar 

  • W J Albery B J Driscoll Y N Kalia (1995) ArticleTitleAmperometric enzyme electrodes Part 8. Enzyme electrodes for choline + betaine aldehyde and hypoxanthine + xanthine. A kinetic model for a one-enzyme sequential substrate system J Electroanal Chem 399 13 Occurrence Handle10.1016/0022-0728(95)04222-9

    Article  Google Scholar 

  • B Leca R M Morelis P R Coulet (1995) ArticleTitleDesign of a choline sensor via direct coating of the transducer by photopolymerization of the sensing layer Sens Actuator B-Chem 27 436 Occurrence Handle10.1016/0925-4005(94)01635-U

    Article  Google Scholar 

  • C A Marquette L J Blum (2003) ArticleTitleSelf-containing reactant biochips for the electrochemiluminescent determination of glucose, lactate and choline Sens Actuator B-Chem 90 112 Occurrence Handle10.1016/S0925-4005(03)00046-7

    Article  Google Scholar 

  • F Qu M Yang J Jiang G Shen R Yu (2005) ArticleTitleAmperometric biosensor for choline based on layer-by-layer assembled functionalized carbon nanotube and polyaniline multilayer film Anal Biochem 344 108 Occurrence Handle10.1016/j.ab.2005.06.007 Occurrence Handle1:CAS:528:DC%2BD2MXos1amtr4%3D

    Article  CAS  Google Scholar 

  • M Yang Y Yang Y Yang G Shen R Yu (2005) ArticleTitleMicrobiosensor for acetylcholine and choline based on electropolymerization/sol-gel derived composite membrane Anal Chim Acta 530 205 Occurrence Handle10.1016/j.aca.2004.09.010 Occurrence Handle1:CAS:528:DC%2BD2MXhtlSgu7c%3D

    Article  CAS  Google Scholar 

  • O N Schuvailo S V Dzyadevych A V El’skaya S Gautier-Sauvigné E Csöregi R Cespuglio A P Soldatkin (2005) ArticleTitleCarbon fibre-based microbiosensors for in vivo measurements of acetylcholine and choline Biosens Bioelectron 21 87 Occurrence Handle10.1016/j.bios.2004.09.017 Occurrence Handle1:CAS:528:DC%2BD2MXlsVCltL0%3D

    Article  CAS  Google Scholar 

  • J J Mitala SuffixJr A C Michael (2006) ArticleTitleImproving the performance of electrochemical microsensors based on enzymes entrapped in a redox hydrogel Anal Chim Acta 556 326 Occurrence Handle10.1016/j.aca.2005.09.053 Occurrence Handle1:CAS:528:DC%2BD28Xhs1yqug%3D%3D

    Article  CAS  Google Scholar 

  • Z Song J Huang B Wu H Shi J Anzai Q Chen (2006) ArticleTitleAmperometric aqueous sol-gel biosensor for low-potential stable choline detection at multi-wall carbon nanotube modified platinum electrode Sens Actuator B-Chem 115 626 Occurrence Handle10.1016/j.snb.2005.10.030

    Article  Google Scholar 

  • A Guerrieri V Lattanzio F Palmisano P G Zambonin (2006) ArticleTitleElectrosynthesized poly(pyrrole)/poly(2-naphthol) bilayer membrane as an effective anti-interference layer for simultaneous determination of acethylcholine and choline by a dual electrode amperometric biosensor Biosens Bioelectron 21 1710 Occurrence Handle10.1016/j.bios.2005.08.005 Occurrence Handle1:CAS:528:DC%2BD28XhsFyiuro%3D

    Article  CAS  Google Scholar 

  • E Tamiya Y Sugiura N E Nepomuceno S Mizoshita K Nakajima A Akiyama I Karube (1991) ArticleTitleUltramicro acetylcholine sensor based on an enzyme-modified carbon fibre electrode Anal Chim Acta 251 129 Occurrence Handle10.1016/0003-2670(91)87125-Q Occurrence Handle1:CAS:528:DyaK38XlsV2iug%3D%3D

    Article  CAS  Google Scholar 

  • T Yao S Handa (2003) ArticleTitleElectroanalytical properties of aldehyde biosensors with a hybrid-membrane composed of an enzyme film and a redox Os-polymer film Anal Sci 19 767 Occurrence Handle10.2116/analsci.19.767 Occurrence Handle1:CAS:528:DC%2BD3sXjvFGhu7g%3D

    Article  CAS  Google Scholar 

  • N Larsson T Ruzgas L Gorton M Kokaia P Kissinger E Csöregi (1998) ArticleTitleDesign and development of an amperometric biosensor for acetylcholine determination in brain microdialysates Electrochim Acta 43 3541 Occurrence Handle10.1016/S0013-4686(98)00102-9 Occurrence Handle1:CAS:528:DyaK1cXlvVWntLo%3D

    Article  CAS  Google Scholar 

  • K Mitsubayashi G Nishio Y Nakayama H Amagai H Watanabe N Jaffrezic-Renault T Noguer J Marty (2005) ArticleTitleBioelectronic sniffers for formaldehyde in the gas phase J Environ Anal Chem 85 917 Occurrence Handle10.1080/03067310500153793 Occurrence Handle1:CAS:528:DC%2BD2MXhtVWgtLvN

    Article  CAS  Google Scholar 

  • T Minamide K Mitsubayashi N Jaffrezic-Renault K Hibi H Endo H Saito (2005) ArticleTitleBioelectronic sniffer with monoamine oxidase for halitosis monitoring Analyst 130 1490 Occurrence Handle10.1039/b506748k Occurrence Handle1:CAS:528:DC%2BD2MXhtV2gsb%2FN

    Article  CAS  Google Scholar 

  • E N Navera M Suzuki K Yokoyama E Tamiya T Takeuchi I Karube (1993) ArticleTitleMicro-choline sensor for acetylcholinesterase determination Anal Chim Acta 281 673 Occurrence Handle10.1016/0003-2670(93)85029-J Occurrence Handle1:CAS:528:DyaK3sXmt1Sis7c%3D

    Article  CAS  Google Scholar 

  • K Chang C Chang S Chou H Han C Chen (2007) ArticleTitleCharacterization of a planar l-glutamate amperometric biosensor immobilized with a photo-crosslinkable polymer membrane Sens Actuator B-Chem 122 195 Occurrence Handle10.1016/j.snb.2006.05.022

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Kanda-Surugadai, Chiyoda-ku, Tokyo, Japan

    Hiroyuki Kudo, Takao Saito, Hirokazu Saito, Kimio Otsuka & Kohji Mitsubayashi

  2. Graduate School of Engineering, Tokai University, Kitakaname, Hiratsuka, Kanagawa, Japan

    Teruyoshi Goto

Authors
  1. Hiroyuki Kudo
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  2. Teruyoshi Goto
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  3. Takao Saito
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  4. Hirokazu Saito
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  5. Kimio Otsuka
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  6. Kohji Mitsubayashi
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Corresponding author

Correspondence to Kohji Mitsubayashi.

Additional information

Correspondence: Kohji Mitsubayashi, Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan

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Kudo, H., Goto, T., Saito, T. et al. Biochemical sniffer with choline oxidase for measurement of choline vapour. Microchim Acta 160, 421–426 (2008). https://doi.org/10.1007/s00604-007-0796-x

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  • DOI: https://doi.org/10.1007/s00604-007-0796-x

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