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Development of a flow injection method for monitoring cell membrane damage of wine lactic acid bacteria

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Abstract.

A flow injection analysis (FIA) system was developed for the determination of phosphate efflux from wine lactic acid bacteria (Oenococus oeni and Lactobacillus hilgardii) as an indication of cell membrane damage. The system allowed the direct injection of the cell suspension, avoiding the filtration step, with minimum sample treatment and minimized reagent consumption. The developed system is characterized by a linear concentration zone between 3.23 × 10−5 and 4.84 × 10−4 mol L−1 PO4 3− and repeatability better than 2.9%. Bacterial suspensions were exposed to a chemical stress with phenolic acids and injected in the FIA system at regular intervals. The extracellular concentration of phosphate was measured spectrophotometrically. The experimental results obtained indicate that hydroxycinnamic acids (p-coumaric, ferulic and caffeic) induced faster phosphate leakage rates than hydroxybenzoic acids (vanillic and p-hydroxybenzoic) in both strains tested, which could be related to their higher lipophilic character.

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References

  • I Ogbomo U Prinzing H-L Schmidt (1990) ArticleTitlePrerequisites for the online control of microbial processes by flow-injection analysis J Biotechnology 14 63 Occurrence Handle10.1016/0168-1656(90)90018-7 Occurrence Handle1:CAS:528:DyaK3cXkslKnu7w%3D

    Article  CAS  Google Scholar 

  • S Benthin J Nielsen J Villadsen (1991) ArticleTitleCharacterisation and application of precise and robust flow-injection analysers for online measurement during fermentations Anal Chim Acta 247 45 Occurrence Handle10.1016/S0003-2670(00)83050-0 Occurrence Handle1:CAS:528:DyaK3MXksVOlur0%3D

    Article  CAS  Google Scholar 

  • K Schügerl (2001) ArticleTitleProgress in monitoring, modelling and control of bioprocesses during the last 20 years J Biotechnology 85 149 Occurrence Handle10.1016/S0168-1656(00)00361-8

    Article  Google Scholar 

  • J Ruzicka E H Hansen (1998) ArticleTitleFlow injection analysis – where are we heading? Trends Anal Chem 2 69 Occurrence Handle10.1016/S0165-9936(97)00118-0

    Article  Google Scholar 

  • M D Johnston G W Hanlon S P Denyer R J W Lambert (2003) ArticleTitleMembrane damage to bacteria caused by single and combined biocides J Appl Microbiol 94 1015 Occurrence Handle10.1046/j.1365-2672.2003.01923.x Occurrence Handle1:CAS:528:DC%2BD3sXltFGqtrc%3D

    Article  CAS  Google Scholar 

  • Denyer S, Hugo W B (1990) Mechanisms of action of chemical biocides, Society of Applied Bacteriology Technical Series no. 27 1st edn. Blackwell Publishing, London, United Kingdom

  • H J Heipieper H Keweloh H J Rehm (1991) ArticleTitleInfluence of phenols on growth and membrane permeability of free and immobilized Escherichia coli Appl Environ Microbiol 57 1213 Occurrence Handle1:CAS:528:DyaK3MXitVens7s%3D

    CAS  Google Scholar 

  • I S Al-Adham A J Dinning I M Eastwood P Austin P J Collier (1998) ArticleTitleCell membrane effects of some common biocides J Ind Microbiol Biot 21 6 Occurrence Handle10.1038/sj.jim.2900554 Occurrence Handle1:CAS:528:DyaK1cXntlKjtLs%3D

    Article  CAS  Google Scholar 

  • C Ohmizo M Yata T Katsu (2004) ArticleTitleBacterial cytoplasmic membrane permeability assay using ion-selective electrodes J Microbiol Meth 59 173 Occurrence Handle10.1016/j.mimet.2004.06.008 Occurrence Handle1:CAS:528:DC%2BD2cXnslGit74%3D

    Article  CAS  Google Scholar 

  • S I Hong Y R Pyun (2001) ArticleTitleMembrane damage and enzyme inactivation of Lactobacillus plantarum by high pressure CO2 treatment Int J Food Microbiol 63 19 Occurrence Handle10.1016/S0168-1605(00)00393-7 Occurrence Handle1:CAS:528:DC%2BD3MXitlOmtg%3D%3D

    Article  CAS  Google Scholar 

  • A J O’Neill K Miller B Oliva I Chopra (2004) ArticleTitleComparison of assays for detection of agents causing membrane damage in Staphylococcus aureus J Antimicrob Chemoth 54 1127 Occurrence Handle10.1093/jac/dkh476 Occurrence Handle1:CAS:528:DC%2BD2cXhtVOjsrjL

    Article  CAS  Google Scholar 

  • K Aronson U Rönner E Borch (2005) ArticleTitleInactivation of Escherichia coli, Listeria innocua and Saccharomyces cerevisiae in relation to membrane permeabilization and subsequent leakage of intracellular compounds due to pulsed electric field processing Int J Food Microbiol 99 19 Occurrence Handle10.1016/j.ijfoodmicro.2004.07.012

    Article  Google Scholar 

  • B Oliva A J O’Neill K Miller W Stubbings I Chopra (2004) ArticleTitleAnti-staphylococcal activity and mode of action of clofazimine J Antimicrob Chemoth 53 435 Occurrence Handle10.1093/jac/dkh114 Occurrence Handle1:CAS:528:DC%2BD2cXhvFSrt7w%3D

    Article  CAS  Google Scholar 

  • M G D Silveira M V San Romão M C Loureiro-Dias F M Rombouts T Abee (2002) ArticleTitleFlow cytometric assessment of membrane integrity of ethanol-stressed Oenococcus oeni cells Appl Environ Microbiol 68 6087 Occurrence Handle10.1128/AEM.68.12.6087-6093.2002

    Article  Google Scholar 

  • H Liu Y Du X Wang L Sun (2004) ArticleTitleChitosan kills bacteria through cell membrane damage Int J Food Microbiol 95 147 Occurrence Handle10.1016/j.ijfoodmicro.2004.01.022 Occurrence Handle1:CAS:528:DC%2BD2cXmt1Whtbg%3D

    Article  CAS  Google Scholar 

  • M C T Diniz O F Filho E V Aquino J J R Rohwedder (2004) ArticleTitleDetermination of phosphate in natural water employing a monosegmented flow system with simultaneous multiple injection Talanta 62 469 Occurrence Handle10.1016/j.talanta.2003.08.016 Occurrence Handle1:CAS:528:DC%2BD2cXjslyktA%3D%3D

    Article  CAS  Google Scholar 

  • J Murphy J P Riley (1962) ArticleTitleA modified single solution method for the determination of phosphate in natural waters Anal Chim Acta 27 31 Occurrence Handle10.1016/S0003-2670(00)88444-5 Occurrence Handle1:CAS:528:DyaF38XksVyntr8%3D

    Article  CAS  Google Scholar 

  • InstitutionalAuthorNameAPHA/AWWA/WEF (1998) Standard methods for the examination of water and wastewater EditionNumber20 American Public Health Association, American Water Works Association, Water Environment Federation Washington DC

    Google Scholar 

  • R Temmerman G Huys J Swings (2004) ArticleTitleIdentification of lactic acid bacteria: culture-dependent and culture-independent methods Trends Food Sci Tech 15 348 Occurrence Handle10.1016/j.tifs.2003.12.007 Occurrence Handle1:CAS:528:DC%2BD2cXjs1Cnt7s%3D

    Article  CAS  Google Scholar 

  • P Ribéreau-Gayon Y Glories A Maujean D Dubourdieu (1999) Handbook of enology – volume 2 – the chemistry of wine stabilization and treatments EditionNumber1 John Wiley & Sons LTD West Sussex

    Google Scholar 

  • M G D Silveira E A Golovina F M R Hoekstra T Abee (2003) ArticleTitleMembrane fluidity adjustments in ethanol-stressed cells Appl Environ Microbiol 10 5826 Occurrence Handle10.1128/AEM.69.10.5826-5832.2003

    Article  Google Scholar 

  • J A Couto T A Hogg (1994) ArticleTitleDiversity of ethanol-tolerant lactobacilli isolated from Douro fortified wine: clustering and identification by numerical analysis of electrophoretic protein profiles J Appl Bacteriology 76 487 Occurrence Handle1:CAS:528:DyaK2cXlslGjsb4%3D

    CAS  Google Scholar 

  • C Reguant A Bordons L Arola N Rozès (2000) ArticleTitleInfluence of phenolic compounds on the physiology of Oenococcus oeni from wine J Appl Microbiol 88 1065 Occurrence Handle10.1046/j.1365-2672.2000.01075.x Occurrence Handle1:CAS:528:DC%2BD3cXksFCjtrk%3D

    Article  CAS  Google Scholar 

  • F M Campos J A Couto T A Hogg (2003) ArticleTitleInfluence of phenolic acids on grow and inactivation of Oenococcus oeni and Lactobacillus hilgardii J Appl Microbiol 94 167 Occurrence Handle10.1046/j.1365-2672.2003.01801.x Occurrence Handle1:CAS:528:DC%2BD3sXhsFOrt7Y%3D

    Article  CAS  Google Scholar 

  • R S Jackson (2000) Wine science – principles, practise, perception EditionNumber2 Academic Press San Diego

    Google Scholar 

  • G McDonnell A D Russell (1999) ArticleTitleAntiseptics and disinfectants: activity, action and resistance Clin Microbiol Rev 12 147 Occurrence Handle1:CAS:528:DyaK1MXpvVOlsQ%3D%3D

    CAS  Google Scholar 

  • InstitutionalAuthorNameIUPAC, International Union of Pure and Applied Chemistry, Analytical Chemistry Division (1976) ArticleTitleCommission on spectrochemical and other optical procedures for analysis. Nomenclature, symbols, units and their usage in spectrochemical analysis. II. Data interpretation Anal Chem 48 2294 Occurrence Handle10.1021/ac50008a068

    Article  Google Scholar 

  • D Huang B Ou R L Prior (2005) ArticleTitleThe chemistry behind antioxidant capacity assays J Agric Food Chem 53 1841 Occurrence Handle10.1021/jf030723c Occurrence Handle1:CAS:528:DC%2BD2MXhslaltLo%3D

    Article  CAS  Google Scholar 

  • F M Campos J A Couto A R Figueiredo I V Tóth A O S S Rangel T A Hogg (2005) ArticleTitleCell membrane damage induced by phenolic acids on wine lactic acid bacteria. Communication presented at the ASEV 56th Annual Meeting 22–24 June 2005, Seattle, Washington Am J Enol Vitic 56 314A

    Google Scholar 

  • M E Ramos-Niño M N Clifford M R Adams (1996) ArticleTitleQuantitative structure activity relationship for the effect of benzoic acids, cinnamic acids and benzaldehydes on Listeria monocytogenes J Appl Bacteriol 80 303

    Google Scholar 

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Authors and Affiliations

  1. Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, P-4200-072, Porto, Portugal

    André F. Torres, Paulo A. R. Mesquita, Francisco M. Campos, José A. Couto, Ildikó V. Tóth, António O. S. S. Rangel & Timothy A. Hogg

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  1. André F. Torres
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  2. Paulo A. R. Mesquita
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  3. Francisco M. Campos
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  4. José A. Couto
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  5. Ildikó V. Tóth
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  6. António O. S. S. Rangel
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  7. Timothy A. Hogg
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Correspondence to António O. S. S. Rangel.

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Torres, A., Mesquita, P., Campos, F. et al. Development of a flow injection method for monitoring cell membrane damage of wine lactic acid bacteria. Microchim Acta 159, 87–93 (2007). https://doi.org/10.1007/s00604-007-0763-6

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  • DOI: https://doi.org/10.1007/s00604-007-0763-6

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