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Interactions of norfloxacin with DNA and determination of DNA at nanogram levels based on the measurement of enhanced resonance light scattering

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Abstract.

A novel assay of DNA at nanogram levels is presented that is based on the measurement of resonance light scattering (RLS) signals in the presence of norfloxacin. The characteristics of RLS spectra, the effective factors and the optimum conditions of the reaction have been investigated. In Britton-Robinson buffer (pH 5.87), norfloxacin has a maximum peak at 405.5 nm and the RLS intensity is greatly enhanced by trace amounts of DNA due to the interaction between norfloxacin and DNA. Mechanistic studies show that the binding of norfloxacin to DNA forms large particles, which result in the significant enhancement of RLS intensity. The enhanced intensity of RLS is proportional to the concentration of DNA in the range from 0.01–2.0 µg mL−1 for yeast DNA, and from 0.02 to 2.3 µg mL−1 for calf thymus DNA. The determination limit (3σ) is 0.7 ng mL−1 for yeast DNA and 1.2 ng mL−1 for calf thymus DNA, respectively. Synthetic samples were determined satisfactorily.

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References

  • G J Gendimenico P L Bouquin K M Tramposch (1988) Anal Biochem 173 45 Occurrence Handle10.1016/0003-2697(88)90156-X Occurrence Handle1:CAS:528:DyaL1cXls1Kltbg%3D

    Article  CAS  Google Scholar 

  • G S Son J A Yeo J M Kim S K Kim H R Moon W Nam (1998) Biophys Chem 74 225 Occurrence Handle10.1016/S0301-4622(98)00178-1 Occurrence Handle1:CAS:528:DyaK1cXmtl2gsLg%3D

    Article  CAS  Google Scholar 

  • A M Maxam W Gilbert (1980) Methods Enzymol 65 499 Occurrence Handle1:CAS:528:DyaL3cXkvFWntb0%3D Occurrence Handle10.1016/S0076-6879(80)65059-9

    Article  CAS  Google Scholar 

  • J B Le Pecq C Paoletti (1966) Anal Biochem 17 100 Occurrence Handle10.1016/0003-2697(66)90012-1 Occurrence Handle1:STN:280:CCiC2svmtFI%3D

    Article  CAS  Google Scholar 

  • J Kapuscinski B Skoczylas (1977) Anal Biochem 83 252 Occurrence Handle10.1016/0003-2697(77)90533-4 Occurrence Handle1:CAS:528:DyaE2sXls1ektr4%3D

    Article  CAS  Google Scholar 

  • H S Rye J M Dabora M A Quesada R A Mathies A N Glazer (1993) Anal Biochem 208 144 Occurrence Handle10.1006/abio.1993.1020 Occurrence Handle1:CAS:528:DyaK3sXpslemtw%3D%3D

    Article  CAS  Google Scholar 

  • R F Pasternack C Bustamante P J Collings A Giannetto E J Gibbs (1993) J Am Chem Soc 115 5393 Occurrence Handle10.1021/ja00066a006 Occurrence Handle1:CAS:528:DyaK3sXksVemsrs%3D

    Article  CAS  Google Scholar 

  • R F Pasternack P J Collings (1995) Science 269 935 Occurrence Handle10.1126/science.7638615 Occurrence Handle1:CAS:528:DyaK2MXnsFOmt7g%3D

    Article  CAS  Google Scholar 

  • C Z Huang K A Li S Y Tong (1996) Anal Chem 68 2259 Occurrence Handle10.1021/ac9511105 Occurrence Handle1:CAS:528:DyaK28Xjt1ehsbk%3D

    Article  CAS  Google Scholar 

  • C Z Huang Y F Li X H Huang M Li (2001) Analyst 125 1267 Occurrence Handle10.1039/b001662o

    Article  Google Scholar 

  • L H Chen Y T Nie L Z Liu H X Shen (2003) Anal Lett 36 107 Occurrence Handle10.1081/AL-120017266 Occurrence Handle1:CAS:528:DC%2BD3sXitlSktrs%3D

    Article  CAS  Google Scholar 

  • Q Wei H Zhang B Du Y Li X X Z (2005) Microchim Acta 151 59 Occurrence Handle10.1007/s00604-005-0375-y Occurrence Handle1:CAS:528:DC%2BD2MXpvV2it7Y%3D

    Article  CAS  Google Scholar 

  • B Bao A G Frutos C Greef J Lahiri U Muller T C Peterson L Warden X Y Xie (2002) Anal Chem 74 1792 Occurrence Handle10.1021/ac0111964 Occurrence Handle1:CAS:528:DC%2BD38Xhslamtb4%3D

    Article  CAS  Google Scholar 

  • G W Song Z X Cai L Li (2004) Microchim Acta 144 23 Occurrence Handle10.1007/s00604-003-0044-y Occurrence Handle1:CAS:528:DC%2BD2cXktVeiuw%3D%3D

    Article  CAS  Google Scholar 

  • Z G Chen W F Ding F L Ren J B Liu Y Z Liang (2005) Anal Chim Acta 550 204 Occurrence Handle10.1016/j.aca.2005.06.057 Occurrence Handle1:CAS:528:DC%2BD2MXhtVSqs7jF

    Article  CAS  Google Scholar 

  • Z G Chen W F Ding Y Z Liang F L Ren Y L Han J B Liu (2005) Microchim Acta 150 35 Occurrence Handle10.1007/s00604-005-0338-3 Occurrence Handle1:CAS:528:DC%2BD2MXktlCitLs%3D

    Article  CAS  Google Scholar 

  • C Q Ma K A Li S Y Tong (1996) Anal Biochem 239 86 Occurrence Handle10.1006/abio.1996.0294 Occurrence Handle1:CAS:528:DyaK28XksFCmtL4%3D

    Article  CAS  Google Scholar 

  • L Y Wang H Q Chen L Li T T Xia L Dong L Wang (2004) Spectrochim Acta A 60 747 Occurrence Handle10.1016/S1386-1425(03)00285-3 Occurrence Handle1:CAS:528:DC%2BD2cXhsVKgtLw%3D

    Article  CAS  Google Scholar 

  • Z G Chen T Y Zhang F L Ren W F Ding (2006) Microchim Acta 153 67

    Google Scholar 

  • X Wu L Li J H Yang Y B Wang S Sun N X Wang (2003) Microchim Acta 141 165 Occurrence Handle10.1007/s00604-002-0944-2 Occurrence Handle1:CAS:528:DC%2BD3sXitVagtL8%3D

    Article  CAS  Google Scholar 

  • C X Yang Y F Li C Z Huang (2002) Anal Bioanal Chem 374 868 Occurrence Handle10.1007/s00216-002-1527-0 Occurrence Handle1:CAS:528:DC%2BD38Xos1yjtLs%3D

    Article  CAS  Google Scholar 

  • S P Liu H Q Luo N B Li Z F Liu W X Zheng (2001) Anal Chem 73 3907 Occurrence Handle10.1021/ac001454h Occurrence Handle1:CAS:528:DC%2BD3MXltVSisb0%3D

    Article  CAS  Google Scholar 

  • C Z Huang P Feng Y F Li K J Tan (2005) Anal Bioanal Chem 382 85 Occurrence Handle10.1007/s00216-005-3192-6 Occurrence Handle1:CAS:528:DC%2BD2MXkt1Sntrw%3D

    Article  CAS  Google Scholar 

  • R F Pasternack K F Schaefer P Hambright (1994) Inorg Chem 33 2062 Occurrence Handle10.1021/ic00087a053 Occurrence Handle1:CAS:528:DyaK2cXivVehtrk%3D

    Article  CAS  Google Scholar 

  • C Z Huang K A Li S Y Tong (1997) Bull Chem Soc Jpn 70 1843 Occurrence Handle10.1246/bcsj.70.1843 Occurrence Handle1:CAS:528:DyaK2sXlsVCgtbk%3D

    Article  CAS  Google Scholar 

  • M S Ibrahim (2001) Anal Chim Acta 443 63 Occurrence Handle10.1016/S0003-2670(01)01184-9 Occurrence Handle1:CAS:528:DC%2BD3MXmtV2gtLc%3D

    Article  CAS  Google Scholar 

  • C Liu X T Chen S Q Li X M Chen (2002) J Chin Chem Anal 30 1218 Occurrence Handle1:CAS:528:DC%2BD38XosFWjt7k%3D

    CAS  Google Scholar 

  • L L Shen A G Pernet (1985) Proc Natl Acad Sci USA 82 307 Occurrence Handle10.1073/pnas.82.2.307 Occurrence Handle1:CAS:528:DyaL2MXhsVarsLw%3D

    Article  CAS  Google Scholar 

  • R T Liu J H Yang X Wu (2002) J Luminescence 96 201 Occurrence Handle10.1016/S0022-2313(01)00234-4 Occurrence Handle1:CAS:528:DC%2BD38XhslymtL4%3D

    Article  CAS  Google Scholar 

  • G S Son J A Yeo M S Kim S K Kim A Holmen B Akerman B Norden (1998) J Am Chem Soc 120 6451 Occurrence Handle10.1021/ja9734049 Occurrence Handle1:CAS:528:DyaK1cXktlWisrw%3D

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Chemistry, Shantou University, 515063, Shantou, China

    Zhanguang Chen, Taiyu Zhang, Xiangming Chen, Yali Han & Li Zhu

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  1. Zhanguang Chen
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  2. Taiyu Zhang
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  3. Xiangming Chen
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  4. Yali Han
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  5. Li Zhu
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Correspondence to Zhanguang Chen.

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Chen, Z., Zhang, T., Chen, X. et al. Interactions of norfloxacin with DNA and determination of DNA at nanogram levels based on the measurement of enhanced resonance light scattering. Microchim Acta 157, 107–112 (2007). https://doi.org/10.1007/s00604-006-0634-6

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  • DOI: https://doi.org/10.1007/s00604-006-0634-6

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