Abstract.
Simple and rapid fluorometric screening methods have been developed based on the competitive binding between the target and an intercalating fluorophore dye to double-stranded-DNA (dsDNA). In this study, the long-wavelength fluorescente dye TOTO-3 was employed as the indicator. Compounds that interact with dsDNA will affect the binding of TOTO-3 to the nucleic acid thereby changing the fluorescence intensity. The analyte concentration is indirectly determined by the decrease in fluorescence intensity. A fiber optic fluorescence screening system was developed for rapid and convenient sample processing. Lambda DNA (48.5 kb) was chosen as a suitable sensing nucleic acid material. Detection of sulfathiazole and chloramphenicol in shrimps using this method was studied in the range of 0.5–25 ng mL−1 of sulfathiazole and of 1–50 ng mL−1 of chloramphenicol. Detection limits of 0.5 ng mL−1 of sulfathiazole and 1 ng mL−1 of chloramphenicol were achieved. This approach is useful as a routine test in the monitoring of antibiotics in the environment or aquaculture products. The easy operation and the rapid and sensitive detection make this a potential high-throughput screening method.
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References
M S Yang M E McGovern M Thompson (1997) Anal Chim Acta 346 259 Occurrence Handle1:CAS:528:DyaK2sXktF2quro%3D
M Mascini I Palchetti G Marrazza (2001) Fresenius J Anal Chem 369 15 Occurrence Handle10.1007/s002160000629 Occurrence Handle1:CAS:528:DC%2BD3MXit1Slsw%3D%3D
M I Pividori A Merkoci S Alegret (2000) Biosens Bioelectron 15 291 Occurrence Handle10.1016/S0956-5663(00)00071-3 Occurrence Handle1:CAS:528:DC%2BD3cXmsFyrsL4%3D
J Wang X Cai G Rivas H Shiraishi N Dontha (1997) Biosens Bioelectron 12 587 Occurrence Handle1:CAS:528:DyaK2sXntF2gsrs%3D Occurrence Handle10.1016/S0956-5663(96)00076-0
J Wang G Rivas X Cai E Palecek P Nielsen H Shiraishi N Dontha D Luo C Parrado M Chicharro P A M Farias F S Valera D H Grant M Ozsoz M N Flair (1997) Anal Chim Acta 347 1 Occurrence Handle10.1016/S0003-2670(96)00598-3 Occurrence Handle1:CAS:528:DyaK2sXksFKitLY%3D
Y C Lin M Y Huang K C Young T T Chang C Y Wu (2000) Sens Actuators B Chem 71 2
K Hashimoto K Ito Y Ishimori (1998) Sens Actuators B Chem 46 220 Occurrence Handle10.1016/S0925-4005(98)00121-X
S Tombelli M Mascini C Sacco A P F Turner (2000) Anal Chim Acta 418 1 Occurrence Handle10.1016/S0003-2670(00)00943-0 Occurrence Handle1:CAS:528:DC%2BD3cXkslWltrw%3D
M A Breimer Y Gelfand O A Sadik (2003) Biosens Bioelectron 18 1135 Occurrence Handle10.1016/S0956-5663(02)00248-8 Occurrence Handle1:CAS:528:DC%2BD3sXkt1artbo%3D
Y Liu B Danielsson (2005) Anal Chem 77 2450 Occurrence Handle1:CAS:528:DC%2BD2MXitVClu7w%3D
Stuart J S, Rupp H S, Hurlbut J A (2003) U.S. food and drug administration laboratory information bulletin. 194
R P Haugland (2002) Handbook of fluorescent probes and research products. Molecular Probes, Inc. U.S. 270
B P Bowen N W Woodbury (2003) Photochem Photobiol 78 582 Occurrence Handle1:CAS:528:DC%2BD2cXhslCn
N Pastor-Navarro C García-Bover Á Maquieira R Puchades (2004) Anal Bioanal Chem 379 1088 Occurrence Handle10.1007/s00216-004-2683-1 Occurrence Handle1:CAS:528:DC%2BD2cXntVehu7g%3D
N R Murtazina S A Eremin O V Mozoleva S J Everest J A Brown R Jackman (2004) Int J Food Sci Technol 39 879 Occurrence Handle1:CAS:528:DC%2BD2cXpvVCgsb0%3D
G Evtugyn A Mingaleva H Budnikov E Stoikova V Vinter S Eremin (2003) Anal Chim Acta 479 125 Occurrence Handle10.1016/S0003-2670(02)01540-4 Occurrence Handle1:CAS:528:DC%2BD3sXhtFCltrc%3D
Commission Decision of 19 September 2001. Off J Eur Commun L251 11
Commission Decision of 30 January 2002. Off J Eur Commun L30 50
J E Roybal (1998) Analytical procedures for drug residues in food of animal origin. Science Technology System West Sacramento, CA 227
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Liu, Y., Danielsson, B. Rapid Fluorometric Screening of Antibiotics in Seafood. Microchim Acta 153, 133–137 (2006). https://doi.org/10.1007/s00604-005-0421-9
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DOI: https://doi.org/10.1007/s00604-005-0421-9