On-Line Inhibited Chemiluminescence Detection of 2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-Glucoside and Baicalin Separated by Capillary Electrophoresis

Abstract.

A capillary electrophoresis on-line inhibited luminol-K₃Fe(CN)₆ chemiluminescence method was employed for the determination of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside (I) and baicalin (baicalein 7-O-glucuronide, II). In this system, luminol was added to running buffer solution and introduced at the head of the separation capillary, and K₃Fe(CN)₆ was introduced at the end of the capillary. The parameters affecting the separation process and CL detection were studied. Under the optimum conditions, the baseline separation of the two analytes was obtained within 4 min. The detection limits (S/N=3) for I and II were 3.0×10⁻⁶ M and 1.2×10⁻⁶ M, respectively. This proposed method was successfully applied to the determination of II in traditional Chinese medicines.