Molecular Imprinting-Chemiluminescence Determination of Norfloxacin Using a Norfloxacin-Imprinted Polymer as the Recognition Material

Abstract.

A selective molecular imprinting-chemiluminescence method was developed for the determination of norfloxacin by using a norfloxacin-imprinted polymer as recognition material and the Ce(IV)-sodium sulfite-norfloxacin chemiluminescence reaction as the detection system. The chemiluminescence intensity responded linearly to the concentration of norfloxacin within 1.0×10⁻⁷∼1.0×10⁻⁵ mol L⁻¹ with a detection limit of 3×10⁻⁸ mol L⁻¹. The relative standard deviation for 5.0×10⁻⁷ mol L⁻¹ norfloxacin solution was 2.4% (n = 7). This method was applied to the determination of norfloxacin in urine samples, and the results obtained were satisfactory.