Polarographic Determination of Lovastatin in the Presence of H₂O₂ in Pharmaceuticals, Human Urine and Serum

Abstract.

The polarographic reduction and catalytic behavior of lovastatin are studied by polarography and cyclic voltammetry. The reduction wave of lovastatin appears at ca. −1.49 V (vs. SCE) in 0.16 mol L⁻¹ Na₂B₄O₇–KH₂PO₄ (pH = 7.4) supporting electrolyte containing 20% ethanol. It is ascribed to a 2e⁻, 2H⁺ addition to the carbonyl group on lactone ring. If H₂O₂ is present, the reduction wave is catalyzed to produce a polarographic catalytic wave. Based on the catalytic wave, a novel method for the determination of lovastatin is proposed. A rectilinear calibration curve of the catalytic wave was obtained for lovastatin concentration in the range 1.5 × 10⁻⁸ to 1.0 × 10⁻⁶ mol L⁻¹. The peak current of the catalytic wave is ca. 12 times higher than that of the corresponding reduction wave. The detection limit is 8.0 × 10⁻⁹ mol L⁻¹. The proposed method is simpler, faster and more sensitive than the known methods for lovastatin analysis, and can be applied to the direct determination of lovastatin in pharmaceuticals, urine and serum without preliminary separation.